A sustainable approach to produce yeast lipid by utilizing marine macroalgae biomass

Marine macroalgae have qualities to utilize it as feedstocks to produce wide spectrum of biofuels, chemicals, and bioactive compounds. Microorganisms play an important role in converting macroalgae biomass into high value products, and genetic modification have been developed to increase their capabilities. The present work has investigated the escalation of lipids by ethyl methanesulfonate (EMS) mutagenesis in oleaginous yeasts and their simultaneous waste-free cultivation in underutilized hydrolyzed macroalgae biomass. After microwave-mediated hydrolysis of the macroalgal biomass, 37 g of glucan per 100 g of biomass were recovered. The examined mutants were further cultivated in microwave-mediated hydrolyzed macroalgae biomass. This study has focused on assessing lipid induction in yeast of EMS mutants. The yeast mutants have shown enhanced maximum biomass (2.1 g/L) with lipid content (47 %).

Automated summary

Marine macroalgae have potential to be used as feedstocks for producing various biofuels, chemicals, and bioactive compounds. Microorganisms play a crucial role in converting macroalgae biomass into valuable products, and genetic modification has been developed to enhance their abilities. This study focused on exploring lipid induction in oleaginous yeasts through ethyl methanesulfonate (EMS) mutagenesis, and cultivating them in hydrolyzed macroalgae biomass.