UV-C light promotes the reductive cleavage of disulfide bonds in β-Lactoglobulin and improves in vitro gastric digestion

beta-Lactoglobulin (beta-Lg) is the main protein in whey and is known for its allergenicity and resistance to the digestion of pepsin and trypsin. The UV-C photoinduced cleavage of disulfide bonds in beta-Lactoglobulin, as promoted by excitation of tryptophan residues (Trp), is shown to induce changes in the protein's secondary structure, significantly reducing the protein's resistance to pepsin digestion. The UV-C light-induced changes in the protein secondary structure are marked by an increase in the contribution of beta-sheet and alpha-helix structures with a concomitantly smaller contribution of the beta-turn structural motif. The photoinduced cleavage of disulfide bonds in beta-Lg has an apparent quantum yield of Phi = 0.0015 +/- 0.0003 and was shown by transient absorption laser flash photolysis to arise by two different pathways: a) the reduction of the disulfide bond Cys(66)Cys(160) occurs by direct electron transfer from the triplet-excited 3Trp to the disulfide bond due to the existence of a CysCys/Trp triad (Cys(66)Cys(160)/Trp(61)) and b) the reduction of the buried Cys(106)Cys(119) disulfide bond involves a reaction with a solvated electron originated by the photoejection of electrons from the triplet-excited (3)Trp decay. The in vitro gastric digestion index for UV-C-treated beta Lg is revealed to have increased significantly by 36 +/- 4 % and 9 +/- 2 % under simulated elderly and young adult digestive conditions, respectively. When compared to the native pro-tein, the peptide mass fingerprint profile of digested UV-C-treated beta-Lg shows a higher content and variety of peptides, including the production of some exclusive bioactive peptides such as PMHIRL and EKFDKALKALPMH.

Automated summary

Beta-lactoglobulin (beta-Lg) is a whey protein known for its allergenicity and resistance to digestion. UV-C light-induced cleavage of disulfide bonds in beta-Lg changes its secondary structure, reducing its resistance to pepsin digestion. The reduction of disulfide bonds occurs through direct electron transfer and reaction with a solvated electron. UV-C-treated beta-Lg showed increased gastric digestion index and production of bioactive peptides.