Protective effect of Paecilomyces cicadae TJJ11213 exopolysaccharide on intestinal mucosa and regulation of gut microbiota in immunosuppressed mice

The exopolysaccharide (EPS) form Paecilomyces cicadae TJJ1213 possessed immunomodulatory activity in vitro, but whether it could regulate the immune system and intestinal microbiota in vivo remained unknown. In this study, the cyclophosphamide (CTX)-induced immunosuppressive mouse model was established to explore the immunomodulatory activity of EPS. Results showed that EPS could increase the immune organ indices, promote the secretion of serum immunoglobulins and up-regulate the expression of cytokines. Additionally, EPS could repair CTX-induced intestinal injury by increasing the expression of tight junction proteins and promoting the production of short-chain fatty acids. Moreover, EPS could remarkably enhance immunity through TLR4/ MyD88/NF-kappa B and mitogen-activated protein kinase (MAPK) signaling pathways. Furthermore, EPS regulated intestinal microbiota by increasing the abundance of beneficial bacteria (Muribaculaceae, Lachnospiraceae NK4A136, Bacteroides, Odoribacter) and reducing the level of harmful bacteria (Alistipes, Helicobacter). In conclusion, our study suggested that EPS had the abilities to enhance immunity, restore intestinal mucosal injury and modulate intestinal microbiota, and may serve as a potential prebiotic to maintain health in the future.

Automated summary

The exopolysaccharide (EPS) from Paecilomyces cicadae TJJ1213 possesses immunomodulatory activity in vitro and can regulate the immune system and intestinal microbiota in vivo. It increases immune organ indices, promotes serum immunoglobulin secretion, and up-regulates cytokine expression. EPS also repairs intestinal injury caused by cyclophosphamide (CTX) by increasing tight junction protein expression and promoting short-chain fatty acid production. Additionally, EPS enhances immunity through TLR4/MyD88/NF-kappa B and mitogen-activated protein kinase (MAPK) signaling pathways and regulates intestinal microbiota by increasing beneficial bacteria and reducing harmful bacteria.