The role of PhoP/PhoQ system in regulating stress adaptation response in Escherichia coli O157:H7

The development of acid tolerance response (ATR) as a result of low pH in Escherichia coli O157:H7 (E. coli O157: H7) contaminating beef during processing is considered a major food safety concern. Thus, in order to explore the formation and molecular mechanisms of the tolerance response of E. coli O157:H7 in a simulated beef processing environment, the resistance of a wild-type (WT) strain and its corresponding Delta phoP mutant to acid, heat, and osmotic pressure was evaluated. Strains were pre-adapted under different conditions of pH (5.4 and 7.0), temperature (37 degrees C and 10 degrees C), and culture medium (meat extract and Luria-Bertani broth media). In addition, the expression of genes related to stress response and virulence was also investigated among WT and Delta phoP strains under the tested conditions. Pre-acid adaptation increased the resistance of E. coli O157:H7 to acid and heat treatment while resistance to osmotic pressure decreased. Moreover, acid adaptation in meat extract medium simulating slaughter environment increased ATR, whereas pre-adaptation at 10 degrees C reduced the ATR. Furthermore, it was shown that mildly acidic conditions (pH = 5.4) and the PhoP/PhoQ two-component system (TCS) acted synergistically to enhance acid and heat tolerance in E. coli O157:H7. Additionally, the expression of genes related to arginine and lysine metabolism, heat shock, and invasiveness was up-regulated, which revealed that the mechanism of acid resistance and cross-protection under mildly acidic conditions was mediated by the PhoP/PhoQ TCS. Both acid adaptation and phoP gene knockout reduced the relative expression of stx1 and stx2 genes which were considered as critical pathogenic factors. Collectively, the current findings indicated that ATR could occur in E. coli O157:H7 during beef processing. Thus, there is an increased food safety risk due to the persistence of tolerance response in the following processing conditions. The present study provides a more comprehensive basis for the effective application of hurdle technology in beef processing.

Automated summary

The formation and molecular mechanisms of acid tolerance response (ATR) in Escherichia coli O157:H7 during beef processing was investigated. Pre-acid adaptation increased resistance to acid and heat, while reducing resistance to osmotic pressure. Acid adaptation in meat extract medium increased ATR, while pre-adaptation at low temperature reduced it. Mildly acidic conditions and the PhoP/PhoQ two-component system acted together to enhance acid and heat tolerance. The expression of genes related to stress response and virulence was up-regulated under acid conditions and mediated by the PhoP/PhoQ TCS. Both acid adaptation and phoP gene knockout reduced the expression of critical pathogenic factors stx1 and stx2. These findings highlight the food safety risk and provide a basis for hurdle technology in beef processing.