4.7 Article

Characterization of the synergistic inhibitory effect of cyanidin-3-O-glucoside and catechin on pancreatic lipase

期刊

FOOD CHEMISTRY
卷 404, 期 -, 页码 -

出版社

ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2022.134672

关键词

Ultrafiltration; Cyanidin-3-O-glucoside; Catechin; Synergistic effect; Pancreatic lipase; Molecular docking

向作者/读者索取更多资源

This study identified novel pancreatic lipase (PL) inhibitors, cyanidin-3-O-glucoside (C3G) and catechin, extracted from black rice and adzuki bean coat extracts. The combination of C3G and catechin showed a synergistic effect in inhibiting PL, and molecular docking analysis revealed that these polyphenols formed interactions with PL amino acid residues. These findings suggest that C3G and catechin have potential for developing food supplements to prevent obesity.
This study aimed to identify novel pancreatic lipase (PL) inhibitors using affinity ultrafiltration combined with spectroscopy and molecular docking. Cyanidin-3-O-glucoside (C3G; IC50: 0.268 mg/mL) and catechin (IC50: 0.280 mg/mL) were shown to be potent PL inhibitors extracted from black rice and adzuki bean coat extracts. Isobologram analysis revealed that the combined use of C3G and catechin at a ratio of 2:3 had a remarkable synergistic effect (IC50 of the mixture: 0.201 mg/mL). The inhibitory mechanism of C3G-catechin mixture was of mixed type. The C3G-catechin mixture had a great impact on PL secondary structures. Molecular docking analysis further demonstrated that these polyphenols formed hydrophobic interactions and hydrogen bonds with amino acid residues in the binding pocket of PL. Collectively, C3G and catechin were shown to inhibit PL in a synergistic manner and can be potentially used for the development of food supplements for obesity prevention.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据