期刊
FOOD CHEMISTRY
卷 404, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2022.134312
关键词
Monofloral honey; Untargeted; targeted mass spectrometry; Characteristic markers; Antioxidant activity; Astragalus membranaceus var; mongholicus Hsiao
This study identified calycosin and formononetin as reliable chemical markers for MH-Am authentication, and determined their contents in MH-Am, other honeys and P-Am through a targeted quantitative analysis. Free radical scavenging assays showed that calycosin directly contributed to the antioxidative activity of MH-Am.
Astragalus membranaceus var. mongholicus Hsiao (Am) is a widely used traditional Chinese herbal medicine. The monofloral honey from Am plant nectar collected by honeybees (MH-Am) has potential medicinal activities. Quality control of MH-Am requires discovery of characteristic markers. In this study, calycosin and formononetin were identified as reliable chemical markers for MH-Am authentication, which were shared with its plant (P-Am), but absent in other honeys based on untargeted mass spectrometry (MS) analysis. The contents of calycosin and formononetin in MH-Am, other honeys and P-Am were determined through a targeted MS-based quantitative approach. Furthermore, free radical scavenging assays showed that calycosin functioned directly in the antioxidative activity of MH-Am. Thus, calycosin has great potential to be certified as a bioactive marker contributing to future quality control of commercial MH-Am products.
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