Effects of three biological combined with chemical methods on the microstructure, physicochemical properties and antioxidant activity of millet bran dietary fibre

The effects of cellulase hydrolysis separately combined with hydroxypropylation, carboxymethylation and phosphate crosslinking on the physicochemical properties and antioxidant activity of millet bran dietary fibre (MBDF) were investigated. Compared to cellulase hydrolysis alone, these dual modifications more effectively improved the soluble fibre content, water-swelling ability, viscosity, emulsifying capacity and cation-exchange capacity of MBDF but reduced the emulsion stability, brightness and polyphenol content of MBDF (P < 0.05). MBDF modified by cellulase hydrolysis combined with hydroxypropylation showed the highest emulsifying capacity (60.03 m2/g) and oil-adsorption capacity (3.32 g/g) but the lowest nitrite ion-adsorbing ability (NIAA). MBDF modified by cellulase hydrolysis with carboxymethylation showed the highest surface hydrophobicity, cation-exchange capacity (0.352 mmol/g) and NIAA (152.89 mu g/g). MBDF modified by cellulase hydrolysis combined with phosphate crosslinking exhibited excellent copper ion-adsorbing ability (19.97 mg/g) and vis-cosity (19.33 cp). Moreover, these dual modifications all enhanced the Fe2+ chelating ability and reducing power of MBDF (P < 0.05).

Automated summary

The effects of cellulase hydrolysis combined with different modifications on the physicochemical properties and antioxidant activity of millet bran dietary fibre were investigated. The results showed that these dual modifications effectively improved the functional properties of MBDF but reduced its emulsion stability, brightness, and polyphenol content. Furthermore, these modifications enhanced the chelating ability and reducing power of MBDF.