Effect of drying technology in Saccharomyces cerevisiae mannans: Structural, physicochemical, and functional properties

Mannans are polysaccharides whose physicochemical and biological properties render them commercialization in several products. Since these properties are strongly dependent on production conditions, the present study aims to assess the impact of different drying technologies - freeze (FDM) and spray drying (SDM) - on the structural, physicochemical, and biological properties of mannans from Saccharomyces cerevisiae. Structural analysis was assessed by FT-IR, PXRD and SEM, whereas physicochemical properties were evaluated based on sugars, protein, ash and water contents, solubility, and molecular weight distribution. Thermal behaviour was analysed by DSC, and antioxidant activity by DPPH and ABTS assays. The parameters which revealed major differences, in terms of structural and physicochemical properties regarded morphology (SEM), physical appearance (colour), moisture (3.6 +/- 0.1 % and 11.9 +/- 0.6 % for FDM and SDM, respectively) and solubility (1 mg/mL for FDM and 25 mg/mL for SDM). Nevertheless, these differences were not translated into the antioxi-dant capacity.

Automated summary

This study evaluates the impact of freeze drying and spray drying on the structural, physicochemical, and biological properties of mannans from Saccharomyces cerevisiae. Structural analysis was conducted using FT-IR, PXRD, and SEM, while physicochemical properties were assessed based on sugars, protein, ash and water contents, solubility, and molecular weight distribution. Thermal behavior was analyzed using DSC, and antioxidant activity was measured using DPPH and ABTS assays.