Effects of pressurized liquid extraction with dimethyl sulfoxide on the recovery of carotenoids and other dietary valuable compounds from the microalgae Spirulina, Chlorella and Phaeodactylum tricornutum

The impact of pressurized liquid extraction (PLE) and DMSO concentration (0, 30, 50, 100 %) on the yield of antioxidants and minerals from Chlorella were investigated. The results showed that PLE increased the antiox-idant yield. Water extracted more proteins, while with 100 % DMSO more polyphenols, chlorophylls, and ca-rotenoids were obtained. The efficiency coefficient (KPLE) results showed that PLE + 100 % DMSO was more suitable for the recovery of antioxidants and pigments from Chlorella (polyphenols 10.465 mg/g, chlorophyll a 6.206 mg/g, chlorophyll b 3.003 mg/g, carotenoids 0.971 mg/g). Thus, PLE + 100 % DMSO was used for re-covery studies on Spirulina, Chlorella, and Phaeodactylum tricornutum. Fucoxanthin, beta-carotene, zeaxanthin, and lutein were the major carotenoids in P. tricornutum, Spirulina, and Chlorella, respectively. Regarding the extraction of minerals, Relative Nutrient Values results were calculated based on Recommended Dietary Al-lowances. The results indicated that the extracts could be used as a mineral source for different populations.

Automated summary

This study investigated the impact of pressurized liquid extraction (PLE) and DMSO concentration on the yield of antioxidants and minerals from Chlorella. The results showed that PLE increased the antioxidant yield, with 100% DMSO obtaining more polyphenols, chlorophylls, and carotenoids. PLE + 100% DMSO was found to be the most suitable method for the recovery of antioxidants and pigments from Chlorella. The extracts were also found to be a good source of minerals for different populations.