Cathepsin H and cathepsin B of Cynoglossus semilaevis are involved in anti-bacterial immunity against Edwardsiella tarda

Cathepsin H and Cathepsin B are two lysosomal cysteine proteases participating in various physiological pro-cesses including immune responses. In fish, the functional roles of Cathepsin H and Cathepsin B during bacterial infection are less understood. In a previous work, we characterized a Cathepsin B homologue (CsCatB) of half -smooth tongue sole (Cynoglossus semilaevis), an economically valuable fish species in China. In this report, we identified a Cathepsin H homologue (CsCatH) from C. semilaevis. In healthy tongue sole, the transcriptional expression of CsCatH was detected in nine different tissues. Laser scanning confocal microscopic analysis showed that ectopically expressed CsCatH and CsCatB were co-localized with the lysosome. Upon infection by Edward-siella tarda, a significant fish pathogen which caused a severe fish disease termed edwardsiellosis, the expressions of CsCatH and CsCatB were remarkedly upregulated. The knockdown of CsCatH and CsCatB significantly increased the replication of E. tarda and mitigated E. tarda-induced apoptosis in tongue sole tissues. These findings revealed the importance of CsCatH and CsCatB in anti-bacterial immunity of tongue sole.

Automated summary

In this study, a lysosomal cysteine protease CsCatH was identified from half-smooth tongue sole. CsCatH and CsCatB were upregulated upon infection by E. tarda, a severe fish pathogen. Knockdown of CsCatH and CsCatB increased the replication of E. tarda and mitigated E. tarda-induced apoptosis in tongue sole tissues. These findings reveal the importance of CsCatH and CsCatB in the anti-bacterial immunity of tongue sole.