Recreating the extracellular matrix: novel 3D cell culture platforms in cancer research

Cancer initiation and progression heavily rely on microenvironmental cues derived from various components of the niche including the extracellular matrix (ECM). ECM is a complex macromolecular network that governs cell functionality. Although the two-dimensional (2D) cell culture systems provide useful information at the molecular level and preclinical testing, they could not accurately represent the in vivo matrix microenvironmental architecture. Hence, it is no surprise that researchers in the last decade have focussed their efforts on establishing novel advanced in vitro culture models that mimic tumour and tissue-specific niches and interactions. These numerous three-dimensional (3D) culture systems that are now widely available, as well as those still under development, grant researchers with new, improved tools to study cancer progression and to explore innovative therapeutic options. Herein, we report on the emerging methods and cutting-edge technologies in 3D cell culture platforms and discuss their potential use in unveiling tumour microenvironmental cues, drug screening and personalized treatment.

Automated summary

Cancer initiation and progression depend on microenvironmental cues, particularly the extracellular matrix (ECM). Two-dimensional (2D) cell culture systems cannot accurately replicate the in vivo matrix microenvironmental architecture. Therefore, researchers have focused on developing advanced three-dimensional (3D) culture models to mimic specific tumor and tissue environments. These 3D culture systems provide improved tools for studying cancer progression and exploring innovative therapies.