4.7 Article

NOTCH signaling inhibition after DAPT treatment exacerbates alveolar echinococcosis hepatic fibrosis by blocking M1 and enhancing M2 polarization

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FASEB JOURNAL
卷 37, 期 5, 页码 -

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WILEY
DOI: 10.1096/fj.202202033R

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alveolar echinococcosis; E. Multilocularis; hepatic fibrosis; macrophage; NOTCH signaling

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In this study, the changes in NOTCH signaling pathway, as well as the fibrotic and inflammatory response of the liver after E. multilocularis infection, were analyzed using liver tissue samples from AE patients and an infected mouse model. It was found that blocking NOTCH signaling pathway exacerbates hepatic fibrosis levels and alters the polarization and origin of hepatic macrophages, suggesting the involvement of NOTCH3/DLL3 pathway in regulating macrophage polarization and fibrosis caused by AE.
Alveolar echinococcosis (AE) is a lethal helminthic liver disease caused by persistent infection with Echinococcus multilocularis (E. multilocularis). Although more and more attention has been paid to the macrophages in E. multilocularis infection, the mechanism of macrophage polarization, a critical player in liver immunity, is seldom studied. NOTCH signaling is involved in cell survival and macrophage-mediated inflammation, but the role of NOTCH signaling in AE has been equally elusive. In this study, liver tissue samples from AE patients were collected and an E. multilocularis infected mouse model with or without blocking NOTCH signaling was established to analyze the NOTCH signaling, fibrotic and inflammatory response of the liver after E. multilocularis infection. Changes in polarization and origin of hepatic macrophages were analyzed by flow cytometry. In vitro qRT-PCR and Western blot assays were performed to analyze key receptors and ligands in NOTCH signaling. Our data demonstrated that hepatic fibrosis develops after AE, and the overall blockade of NOTCH signaling caused by DAPT treatment exacerbates the levels of hepatic fibrosis and alters the polarization and origin of hepatic macrophages. Blocking NOTCH signaling in macrophages after E. multilocularis infection downregulates M1 and upregulates M2 expression. The downregulation of NTCH3 and DLL-3 in the NOTCH signaling pathway is significant. Therefore, NOTCH3/DLL3 may be the key pathway in NOTCH signaling regulating macrophage polarization affecting fibrosis caused by AE.

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