期刊
EXPERIMENTAL CELL RESEARCH
卷 425, 期 2, 页码 -出版社
ELSEVIER INC
DOI: 10.1016/j.yexcr.2023.113528
关键词
Propionate; Endothelial cell-selective adhesion molecule; Caco-2 cells; Intestinal barrier; Tight junction
This study investigated the role of propionate, a major short-chain fatty acid produced by intestinal microorganisms, in the regulation of the intestinal tight junction (TJ) barrier. It was found that propionate strengthened the TJ barrier integrity in human intestinal Caco-2 cells, as indicated by decreased permeability to macromolecules and increased transepithelial electrical resistance. Propionate upregulated ESAM, a TJ-associated protein, and this upregulation was confirmed using various analyses such as qRT-PCR and immunoblotting.
Regulation of the intestinal barrier is closely associated with intestinal microbial metabolism. This study investigated the role of propionate, a major short-chain fatty acid produced by intestinal microorganisms, in the regulation of the tight junction (TJ) barrier in human intestinal Caco-2 cells. Propionate strengthened TJ barrier integrity, as indicated by decreased permeability to macromolecules and increased transepithelial electrical resistance in Caco-2 cells. DNA microarray analysis revealed that propionate upregulated endothelial cell -selective adhesion molecule (ESAM), a TJ-associated protein, without any increase in other TJ proteins. The upregulation of ESAM was confirmed using quantitative reverse transcription-PCR, immunoblotting, and immunofluorescence analyses. Luciferase promoter analysis demonstrated that propionate induced the tran-scriptional activation of ESAM. The effects of propionate were sensitive to nilotinib inhibition of NR2C2. Overexpression of human ESAM (hESAM) in canine kidney epithelial MDCK-II cells lowered the permeability to macromolecules in a manner similar to that of propionate-treated Caco-2 cells. hESAM overexpression facilitated calcium-induced assembly of the TJ complex in MDCK-II cells. Taken together, propionate strengthened the intestinal TJ barrier by increasing ESAM levels in Caco-2 cells.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据