期刊
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
卷 249, 期 3, 页码 739-748出版社
SPRINGER
DOI: 10.1007/s00217-022-04169-7
关键词
Nelumbo nucifera Gaertn; leaves; Alkaloids; RAW 264; 7 macrophages; Anti-inflammation; High-performance liquid chromatography analysis
Lotus leaves contain valuable functional alkaloids that have inhibitory effects on free radicals and nitric oxide production. Five alkaloids were isolated and identified, with different compounds showing different activities. Compound 5, which accounted for a significant portion of total alkaloids, exhibited the highest DPPH radical-scavenging effect and also inhibited NO production through suppressing iNOS expression. The study suggests that alkaloid extracts from lotus leaves can be used as functional ingredients.
Lotus (Nelumbo nucifera Gaertn.) is an aquatic perennial; and lotus leaves were used for food and functional herbal tea in Asia, while alkaloids are regarded as important components. Total alkaloids (TAs), phenolic alkaloids (PAs), and non-PAs from lotus leaves revealed inhibitory effects on 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals scavenging effects and nitric oxide (NO) production in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages. Five alkaloids were isolated and identified as asimilobine (1), roemerine (2), lysicamine (3), nuciferine (4), and N-methylcoclaurine (5). Of them, compounds 1 and 2 exhibited potency as direct NO scavengers while 5 exerted the highest DPPH radical-scavenging effect. Compound 5 also inhibited NO production with an IC50 value of 6 mu M through suppressing inducible NO synthase (iNOS) expression in LPS-induced RAW 264.7 macrophages. Compounds 2 and 3 inhibited NO production (IC50 values of 21 and 25 mu M, respectively) as well as iNOS and cyclooxygenase (COX)-2 expressions. Further, compound 5 occupied 27.5% of TAs by high-performance liquid chromatography (HPLC) analysis, which was mainly responsible for the activities. Besides, no obvious cytotoxicity was observed under treated concentrations. The present study suggests that alkaloid extracts and individual components can be valuable functional ingredients from lotus leaves.
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