Silicon Limitation Impairs the Tolerance of Marine Diatoms to Pristine Microplastics

Marine diatoms are currently facing increasing threats from microplastic (MP) pollution that is intertwined with the disturbed nutrient stoichiometry in seawater. The effects of nutrient imbalances such as silicon (Si) limitation on the interactions between diatoms and MPs remain poorly understood. In contrast to previous studies which mainly focused on MP toxicity, this study emphasizes how Si availability affects nano-scale interactions between pristine polystyrene MPs and diatom surfaces. Results showed that Si-starved cells were less tolerant to MP toxicity than the Si-enriched counterparts. Si limitation significantly changed the configuration and chemical composition of the perforated frustules, forming less negatively charged, more adhesive, and mechanically weaker cells. All of these changes facilitated the adsorption and hetero-aggregation between the diatom cells and MPs and compromised the diatoms' resistance to MP attack. Our study provides novel insights into the effects of pristine MPs in the marine environment under the context of dynamic nutrient conditions.

Automated summary

Marine diatoms are facing increasing threats from microplastic pollution, especially under disturbed nutrient stoichiometry in seawater. This study focuses on the effects of silicon limitation on the interactions between diatoms and pristine polystyrene microplastics. The results showed that Si-starved cells were less tolerant to microplastic toxicity due to changes in cell configuration and composition. These changes facilitated adsorption and aggregation between diatoms and microplastics, compromising diatoms' resistance. This study provides new insights into the effects of microplastics in dynamic nutrient conditions in the marine environment.