期刊
ELECTROANALYSIS
卷 -, 期 -, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.202300012
关键词
amperometry; biosensor; dopamine; laccase; nanowires
One-dimensional Ni/Au/PPy-COOH nanowires with multiple segments were synthesized and their smooth surfaces and magnetic properties were investigated. These nanowires were used to modify the electrode surface and as a micro-environment for Trametes versicolor laccase. The biosensor based on this design demonstrated high sensitivity, excellent selectivity, wide linear range, and low LOD for the detection of dopamine in biological samples.
One-dimensional Ni/Au/PPy-COOH nanowires with multiple segments were synthesized in this study. Smooth surfaces and magnetic properties of nanowires were investigated by scanning transmission electron microscopy (SEM), Energy-dispersive X-ray spectroscopy (EDX), and Electron Spin Resonance (ESR) techniques. The nanowires were used to modify the screen-printed electrode surface and as a micro-environment for Trametes versicolor laccase. The ability of this enzyme biosensor to detect dopamine change in human biological samples was demonstrated by a wide linear range (0.01-50 mu M) and a low LOD (2.265 nM). In addition, the biosensor exhibited excellent selectivity allowing the detection of dopamine in the presence of ascorbic acid, uric acid, L-Cys, serotonin, and glucose, with high sensitivity of reduction currents obtained at -0.2 V (vs. Ag/AgCl). The proposed biosensor allowed the detection of dopamine in commercial serum and artificial urine with recovery values close to 100 %. It also demonstrated reproducibility, reusability, and long-term storage stability. The sensitivity, K-m(app), and I-max values of the biosensor were determined as 2.05 mu M and 1.03 mu A, respectively. The LAC-Ni/Au/PPy-COOH/NAF/SPE biosensor is a reliable design for detecting dopamine with a wide linear range.
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