Strong chemiluminescence intensities of bis-acridinium esters under neutral and alkaline conditions

Four novel bis-acridinium esters were synthesized using common methods, and their chemiluminescence properties were investigated at pH 7-10. In the chemiluminescence of bis-acridinium esters, the introduction of fluorine at the 2-position of the phenyl ring and methyl groups at the 2,7-positions of the acridine ring changed greatly the chemiluminescence intensities. Pentane-1,5-diyl bis(2-fluoro-4-(2,7,10-trimethylacridinium-9-car-bonyloxy)benzoate) bis(trifluoromethanesulfonate) showed strong chemiluminescence and long-lasting chem-iluminescence under both neutral and alkaline conditions. Pentane-1,5-diyl bis(2-fluoro-4-(10-methylacridinium-9-carbonyloxy)benzoate) bis(trifluoromethanesulfonate) was applied for highly sensitive chemiluminescence determination of hydrogen peroxide and catalase activity. The linear calibration ranges of hydrogen peroxide and catalase activity were 5-1000 mu M (R = 0.999) and 0.05-1 U/mL (R = 0.998), respectively.

Automated summary

Four novel bis-acridinium esters were synthesized and their chemiluminescence properties were studied. The introduction of fluorine and methyl groups at specific positions greatly affected the chemiluminescence intensities. One of the esters showed strong and long-lasting chemiluminescence under neutral and alkaline conditions and was used for sensitive determination of hydrogen peroxide and catalase activity. The linear calibration ranges for hydrogen peroxide and catalase activity were 5-1000 μM and 0.05-1 U/mL, respectively.