期刊
DNA REPAIR
卷 126, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.dnarep.2023.103488
关键词
Rrm3; Rad5; Mus81; Helicase; Replication fork restart; Chromosomal rearrangements; Recombinogenic DNA lesions
To ensure genome stability, replication progress needs to be monitored and accurately completed. In the yeast Saccharomyces cerevisiae, the DNA helicase Rrm3, belonging to the PIF1 family, facilitates replication fork progression through an unknown mechanism. Disrupting Rrm3 helicase activity leads to increased replication fork pausing in the yeast genome.
The genome must be monitored to ensure its duplication is completed accurately to prevent genome instability. In Saccharomyces cerevisiae, the 5 ' to 3 ' DNA helicase Rrm3, a member of the conserved PIF1 family, facilitates replication fork progression through an unknown mechanism. Disruption of Rrm3 helicase activity leads to increased replication fork pausing throughout the yeast genome. Here, we show that Rrm3 contributes to replication stress tolerance in the absence of the fork reversal activity of Rad5, defined by its HIRAN domain and DNA helicase activity, but not in the absence of Rad5 ' s ubiquitin ligase activity. The Rrm3 and Rad5 helicase activities also interact in the prevention of recombinogenic DNA lesions, and DNA lesions that accumulate in their absence need to be salvaged by a Rad59-dependent recombination pathway. Disruption of the structurespecific endonuclease Mus81 leads to accumulation of recombinogenic DNA lesions and chromosomal rearrangements in the absence of Rrm3, but not Rad5. Thus, at least two mechanisms exist to overcome fork stalling at replication barriers, defined by Rad5-mediated fork reversal and Mus81-mediated cleavage, and contribute to the maintenance of chromosome stability in the absence of Rrm3.
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