Sensitivity of the PBP2a SA Culture Colony Test on shortly incubated subcultures of methicillin-resistant staphylococci from positive blood cultures

The sensitivity of an immunochromatographic assay for detecting methicillin resistance (PBP2a SA Culture Colony Test, Alere-Abbott) on shortly incubated subcultures of staphylococci in blood cultures was evalu-ated. The assay is highly sensitive for the detection of methicillin-resistant Staphylococcus aureus after 4 hour-subculture but requires 6 hour-incubation for methicillin-resistant coagulase-negative staphylococci.(c) 2023 Elsevier Inc. All rights reserved.

Automated summary

The sensitivity of an immunochromatographic assay for detecting methicillin resistance in short-term subcultures of staphylococci in blood cultures was evaluated. The assay showed high sensitivity for detecting methicillin-resistant Staphylococcus aureus after 4 hours of subculture, but required 6 hours of incubation for methicillin-resistant coagulase-negative staphylococci. (c) 2023 Elsevier Inc. All rights reserved.