Memory deficits in a juvenile rat model of type 1 diabetes are due to excess 11 beta-HSD1 activity, which is upregulated by high glucose concentrations rather than insulin deficiency

Aims/hypothesis Children with diabetes may display cognitive alterations although vascular disorders have not yet appeared. Variations in glucose levels together with relative insulin deficiency in treated type 1 diabetes have been reported to impact brain function indirectly through dysregulation of the hypothalamus-pituitary-adrenal axis. We have recently shown that enhancement of glucocorticoid levels in children with type 1 diabetes is dependent not only on glucocorticoid secretion but also on glucocorticoid tissue concentrations, which is linked to 11 beta-hydroxysteroid dehydrogenase type 1 (11 beta-HSD1) activity. Hypothalamus-pituitary-adrenal axis dysfunction and memory alteration were further dissected in a juvenile rat model of diabetes showing that excess 11 beta-HSD1 activity within the hippocampus is associated with hippocampal-dependent memory deficits. Here, to investigate the causal relationships between diabetes, 11 beta-HSD1 activity and hippocampus-dependent memory deficits, we evaluated the beneficial effect of 11 beta-HSD1 inhibition on hippocampal-related memory in juvenile diabetic rats. We also examined whether diabetes-associated enhancement of hippocampal 11 beta-HSD1 activity is due to an increase in brain glucose concentrations and/or a decrease in insulin signalling. Methods Diabetes was induced in juvenile rats by daily i.p. injection of streptozotocin for 2 consecutive days. Inhibition of 11 beta-HSD1 was obtained by administrating the compound UE2316 twice daily by gavage for 3 weeks, after which hippocampal-dependent object location memory was assessed. Hippocampal 11 beta-HSD1 activity was estimated by the ratio of corticosterone/dehydrocorticosterone measured by LC/MS. Regulation of 11 beta-HSD1 activity in response to changes in glucose or insulin levels was determined ex vivo on acute brain hippocampal slices. The insulin regulation of 11 beta-HSD1 was further examined in vivo using virally mediated knockdown of insulin receptor expression specifically in the hippocampus. Results Our data show that inhibiting 11 beta-HSD1 activity prevents hippocampal-related memory deficits in diabetic juvenile rats. A significant increase (53.0 +/- 9.9%) in hippocampal 11 beta-HSD1 activity was found in hippocampal slices incubated in high glucose conditions (13.9 mmol/l) vs normal glucose conditions (2.8 mmol/l) without insulin. However, 11 beta-HSD1 activity was not affected by variations in insulin concentration either in the hippocampal slices or after a decrease in hippocampal insulin receptor expression. Conclusions/interpretation Together, these data demonstrate that an increase in 11 beta-HSD1 activity contributes to memory deficits observed in juvenile diabetic rats and that an excess of hippocampal 11 beta-HSD1 activity stems from high glucose levels rather than insulin deficiency. 11 beta-HSD1 might be a therapeutic target for treating cognitive impairments associated with diabetes.

Automated summary

Diabetes can affect the cognitive abilities of children although vascular disorders have not yet appeared. Dysregulation of the hypothalamus-pituitary-adrenal axis due to variations in glucose levels and relative insulin deficiency in treated type 1 diabetes can indirectly impact brain function. Inhibiting the activity of 11 beta-hydroxysteroid dehydrogenase 1 (11 beta-HSD1) can prevent memory deficits in diabetic juvenile rats.