期刊
DEVELOPMENTAL NEUROBIOLOGY
卷 83, 期 3-4, 页码 73-90出版社
WILEY
DOI: 10.1002/dneu.22910
关键词
brain development; CRISPR; migration; neural stem cells; neuron; radial glia
In utero electroporation (IUE) is a technique that allows transfection of neurons and neural progenitors in the embryonic brain, enabling continued development and analysis of neural development.
In utero electroporation (IUE) is a technique developed in the early 2000s to transfect the neurons and neural progenitors of embryonic brains, thus enabling continued development in utero and subsequent analyses of neural development. Early IUE experiments focused on ectopic expression of plasmid DNA to analyze parameters such as neuron morphology and migration. Recent advances made in other fields, such as CRISPR/CAS9 genome editing, have been incorporated into IUE techniques as they were developed. Here, we provide a general review of the mechanics and techniques involved in IUE and explore the breadth of approaches that can be used in conjunction with IUE to study cortical development in a rodent model, with a focus on the novel advances in IUE techniques. We also highlight a few cases that exemplify the potential of IUE to study a broad range of questions in neural development.
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