The maturation and aging trajectory of Marchantia polymorpha at single-cell resolution

Bryophytes represent a sister to the rest of land plants. Despite their evolutionary importance and relatively simple body plan, a comprehensive understanding of the cell types and transcriptional states that underpin the temporal development of bryophytes has not been achieved. Using time-resolved single-cell RNA sequencing, we define the cellular taxonomy of Marchantia polymorpha across asexual reproduction phases. We identify two maturation and aging trajectories of the main plant body of M. polymorpha at single-cell resolution: the gradual maturation of tissues and organs along the tip-to-base axis of the midvein and the progressive decline of meristem activities in the tip along the chronological axis. Specifically, we observe that the latter aging axis is temporally correlated with the formation of clonal propagules, suggesting an ancient strategy to optimize allocation of resources to producing offspring. Our work thus provides insights into the cellular heterogeneity that underpins the temporal development and aging of bryophytes.

Automated summary

Using time-resolved single-cell RNA sequencing, the cellular taxonomy of Marchantia polymorpha during asexual reproduction phases is defined. Two maturation and aging trajectories of the main plant body are identified: gradual maturation of tissues and organs along the tip-to-base axis of the midvein and progressive decline of meristem activities in the tip along the chronological axis. The observed aging trajectory is temporally correlated with the formation of clonal propagules, suggesting an ancient strategy to optimize resource allocation for producing offspring.