LncRNA GAS5 as an Inflammatory Regulator Acting through Pathway in Human Lupus

Aim To investigate the contribution of GAS5 in the pathogenesis of SLE. Background Systemic Lupus Erythematosus (SLE) is characterized by aberrant activity of the immune system, leading to variable clinical symptoms. The etiology of SLE is multifactor, and growing evidence has shown that long noncoding RNAs (lncRNAs) are related to human SLE. Recently, lncRNA growth arrest-specific transcript 5 (GAS5) has been reported to be associated with SLE. However, the mechanism between GAS5 and SLE is still unknown. Objective Find the specific mechanism of action of lncRNA GAS5 in SLE. Methods Collecting samples of the SLE patients, Cell culture and treatment, Plasmid construction, and transfection, Quantitative real-time PCR analysis, Enzyme-linked immunosorbent assay (ELISA), Cell viability analysis, Cell apoptosis analysis, Western blot. Results In this research, we investigated the contribution of GAS5 in the pathogenesis of SLE. We confirmed that, compared to healthy people, the expression of GAS5 was significantly decreased in peripheral monocytes of SLE patients. Subsequently, we found that GAS5 can inhibit the proliferation and promote the apoptosis of monocytes by over-expressing or knocking down the expression of GAS5. Additionally, the expression of GAS5 was suppressed by LPS. Silencing GAS5 significantly increased the expression of a group of chemokines and cytokines, including IL-1 & beta;, IL-6, and THF & alpha;, which were induced by LPS. Furthermore, it was identified the involvement of GAS5 in the TLR4-mediated inflammatory process was through affecting the activation of the MAPK signaling pathway. Conclusion In general, the decreased GAS5 expression may be a potential contributor to the elevated production of a great number of cytokines and chemokines in SLE patients. And our research suggests that GAS5 contributes a regulatory role in the pathogenesis of SLE, and may provide a potential target for therapeutic intervention.

Automated summary

The contribution of long noncoding RNA-GAS5 in the pathogenesis of systemic lupus erythematosus (SLE) was investigated. It was found that the expression of GAS5 was significantly decreased in peripheral monocytes of SLE patients compared to healthy individuals. GAS5 was shown to inhibit monocyte proliferation and promote apoptosis. The study also revealed the involvement of GAS5 in the TLR4-mediated inflammatory process through the MAPK signaling pathway.