NMR of RNA- Structure and interactions

RNA was shown to have a more substantial role in the regu-lation of diverse cellular processes than anticipated until recently. Answers to questions what is the structure of specific RNAs, how structure changes to accommodate different functional roles, and how RNA senses other biomolecules and changes its fold upon interaction create a complete repre-sentation of RNA involved in cellular processes. Nuclear magnetic resonance (NMR) spectroscopy encompasses a collection of methods and approaches that offer insight into several structural aspects of RNAs. We review the most recent advances in the field of viral, long non-coding, regulatory, and four-stranded RNAs, with an emphasis on the detection of dynamic sub-states and in view of chemical modifications that expand RNA's function.

Automated summary

Recent research has shown that RNA plays a more significant role in cellular processes regulation than previously anticipated. Understanding the structure of specific RNAs, how the structure changes to accommodate different functional roles, and how RNA interacts with other biomolecules and changes its fold are important for a complete representation of RNA's involvement in cellular processes. Nuclear magnetic resonance (NMR) spectroscopy is a collection of methods and approaches that provide insights into various structural aspects of RNAs. This review focuses on the recent advances in viral, long non-coding, regulatory, and four-stranded RNAs, with a particular emphasis on detecting dynamic sub-states and the impact of chemical modifications on RNA's function.