Hyperpolarized NMR metabolomics

Hyperpolarized NMR is a promising approach to address the sensitivity limits of conventional NMR metabolomics approaches, which currently fails to detect minute metabolite concentrations in biological samples. This review describes how tremendous signal enhancement offered by dissolutiondynamic nuclear polarization and parahydrogen-based techniques can be fully exploited for molecular omics sciences. Recent developments, including the combination of hyperpolarization techniques with fast multi-dimensional NMR implementation and quantitative workflows are described, and a comprehensive comparison of existing hyperpolarization techniques is proposed. High-throughput, sensitivity, resolution and other relevant challenges that should be tackled for a general application of hyperpolarized NMR in metabolomics are discussed.

Automated summary

Hyperpolarized NMR is a promising method to overcome the limited sensitivity of conventional NMR metabolomics in detecting minute metabolite concentrations in biological samples. This review discusses the utilization of dissolution dynamic nuclear polarization and parahydrogen-based techniques for molecular omics sciences, as well as recent advancements in combining hyperpolarization with fast multi-dimensional NMR and quantitative workflows. The review also proposes a comprehensive comparison of existing hyperpolarization techniques and addresses challenges in high throughput, sensitivity, and resolution for the general application of hyperpolarized NMR in metabolomics.