期刊
CURRENT NEUROPHARMACOLOGY
卷 21, 期 6, 页码 1433-1449出版社
BENTHAM SCIENCE PUBL LTD
DOI: 10.2174/1570159X21666230303123555
关键词
Intrasynaptic mitochondria; density gradient centrifugation; mitochondrial membrane potential (MMP); electron transport chain (ETC); calcium capacitance; reactive oxygen species (ROS); glutathione (GSH); Trolox equivalent antioxidant capacity (TEAC)
Mitochondria play important roles in neuronal development, physiology, plasticity, and pathology through their regulation of bioenergetic, calcium, redox, and cell survival/death signaling. However, a comprehensive discussion on the relevance of isolated brain mitochondria in neuroscience research has been lacking. This mini-review explores the commonly used analytical assays for assessing mitochondrial physiology and dysfunction in organello, with a focus on neuroscience research. It also provides protocols for isolating mitochondria, assessing their quality, and preserving them for future use.
Mitochondria regulate multiple aspects of neuronal development, physiology, plasticity, and pathology through their regulatory roles in bioenergetic, calcium, redox, and cell survival/death signalling. While several reviews have addressed these different aspects, a comprehensive discussion focussing on the relevance of isolated brain mitochondria and their utilities in neuroscience research has been lacking. This is relevant because the employment of isolated mitochondria rather than their in situ functional evaluation, offers definitive evidence of organelle-specificity, negating the interference from extra mitochondrial cellular factors/signals. This mini-review was designed primarily to explore the commonly employed in organello analytical assays for the assessment of mitochondrial physiology and its dysfunction, with a particular focus on neuroscience research. The authors briefly discuss the methodologies for biochemical isolation of mitochondria, their quality assessment, and cryopreservation. Further, the review attempts to accumulate the key biochemical protocols for in organello assessment of a multitude of mitochondrial functions critical for neurophysiology, including assays for bioenergetic activity, calcium and redox homeostasis, and mitochondrial protein translation. The purpose of this review is not to examine each and every method or study related to the functional assessment of isolated brain mitochondria, but rather to assemble the commonly used protocols of in organello mitochondrial research in a single publication. The hope is that this review will provide a suitable platform aiding neuroscientists to choose and apply the required protocols and tools to address their particular mechanistic, diagnostic, or therapeutic question dealing within the confines of the research area of mitochondrial patho-physiology in the neuronal perspective.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据