期刊
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
卷 28, 期 3, 页码 399-408出版社
AMER CHEMICAL SOC
DOI: 10.1007/s13361-016-1557-z
关键词
TOF-SIMS; Matrix enhancement; Graphene oxide; Molecular ions; Bio-imaging
资金
- National Natural Science Foundation of China [21390410]
- 973 program [2013CB933804]
- Ministry of Science and Technology of China [2012YQ12006003]
- National Natural Science Foundation [21327902]
Using matrix to enhance the molecular ion signals for biomolecule identification without loss of spatial resolution caused by matrix crystallization is a great challenge for the application of TOF-SIMS in real-world biological research. In this report, graphene oxide (GO) was used as a matrix for TOF-SIMS to improve the secondary ion yields of intact molecular ions ([M + H](+)). Identifying and distinguishing the molecular ions of lipids (m/z > 700) therefore became straightforward. The spatial resolution of TOF-SIMS imaging could also be improved as GO can form a homogeneous layer of matrix instead of crystalline domain, which prevents high spatial resolution in TOF-SIMS imaging. Lipid mapping in presence of GO revealed the delicate morphology and distribution of single vesicles with a diameter of 800 nm. On GO matrix, the vesicles with similar shape but different chemical composition could be distinguished using molecular ions. This novel matrix holds potentials in such applications as the analysis and imaging of complex biological samples by TOF-SIMS.
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