期刊
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
卷 27, 期 9, 页码 1575-1578出版社
SPRINGER
DOI: 10.1007/s13361-016-1422-0
关键词
Mass spectrometry imaging; MALDI; Matrix; Recrystallization; Maize; Lipids; High spatial resolution
资金
- US Department of Energy (DOE), Office of Basic Energy Sciences, Division of Chemical Sciences, Geosciences, and Biosciences
- DOE [DE-AC02-07CH11358]
Matrix recrystallization is optimized and applied to improve lipid ion signals in maize embryos and leaves. A systematic study was performed varying solvent and incubation time. During this study, unexpected side reactions were found when methanol was used as a recrystallization solvent, resulting in the formation of a methyl ester of phosphatidic acid. Using an optimum recrystallization condition with isopropanol, there is no apparent delocalization demonstrated with a transmission electron microscopy (TEM) pattern and maize leaf images obtained at 10 mu m spatial resolution.
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