期刊
CONTACT DERMATITIS
卷 88, 期 6, 页码 425-437出版社
WILEY
DOI: 10.1111/cod.14316
关键词
allergic contact dermatitis; CXCL2; epidermal-resident memory T cells; neutrophils
This study reveals that clinically relevant contact allergens can generate pathogenic, epidermal CD8(+) T-RM cells that recruit neutrophils following re-exposure to the allergen, but this response is normally suppressed by the induction of anti-inflammatory CD4(+) T cells.
Background CD8(+) epidermal-resident memory T (T-RM) cells play central roles in local flare-up responses to experimental contact allergens by inducing massive influx of neutrophils to the epidermis upon allergen challenge. Whether similar immunopathogenic mechanisms are involved in the responses to clinically relevant contact allergens is unknown.Methods The immune response to cinnamal, ?-phenylenediamine (PPD) and methylisothiazolinone (MI) was studied in a well-established mouse model for allergic contact dermatitis that includes formation of T-RM cells by ELISA, flow cytometry, fluorescence microscopy analyses and cell depletion protocols.Results We show that the formation of CD4(+) and CD8(+) epidermal T-RM cells and the inflammatory response are highly allergen-dependent. However, the magnitude of the flare-up responses correlated with the number of epidermal CD8(+) T-RM cells, CXCL1/CXCL2 release and recruitment of neutrophils to the epidermis. Finally, depletion of CD4(+) T cells strongly enhanced the number of epidermal CD8(+) T-RM cells, the flare-up response and the epidermal infiltration of neutrophils for all allergens.Conclusion As the first, this study demonstrates that clinically relevant contact allergens have the ability to generate pathogenic, epidermal CD8(+) T-RM cells that recruit neutrophils following re-exposure to the allergen, but that this normally is counteracted by the simultaneous induction of anti-inflammatory CD4(+) T cells.
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