Multixenobiotic defence mechanism in native and exotic freshwater snails as a biomarker for land uses-changes

Human activities such as agriculture and urbanization generate a large number of substances like personal care products, pharmaceutical compounds, and pesticides, which often reach aquatic environments and represent a threat to biodiversity. Many organisms have developed different evolutionary strategies to remove pervasive substances from their bodies, allowing them to persist even in polluted environments, and one of these is the multixenobiotic resistance (MXR) mechanism associated with the expression of membrane proteins like P-glycoprotein (P-gp). Numerous chemical compounds with diverse functions and structures can modulate this mechanism, which can be employed as a pollution biomarker. We examined the MXR activity in two species of snails that inhabit Patagonian freshwaters. Functional assay measurements of MXR were conducted on the native Chilina dombeiana and the exotic Physella acuta in stream reaches affected by anthropogenic impacts. Results indicated that at agricultural sites, C. dombeiana snails had a more active MXR system than organisms sampled at reference and moderately disturbed urban sites, whereas P. acuta snails from agricultural and highly disturbed urban sites showed better detoxifying activity than organisms from reference sites. Only in exotic snails, part of this activity was due to the action of P-gp. The most important environmental variables explaining MXR activity were ammonium, nitrate and nitrite, phosphates, and electrical conductivity. These results show the promise of measuring MXR activity in native and exotic snails, as a biomarker in the environmental monitoring of Pata-gonian freshwaters.

Automated summary

Human activities generate substances that threaten aquatic biodiversity, but many organisms have developed strategies like the MXR mechanism to remove these substances. This study examined MXR activity in two species of snails in Patagonian freshwaters and found that native snails had higher MXR activity at agricultural sites, while exotic snails showed better detoxifying activity at agricultural and highly disturbed urban sites. Environmental variables such as ammonium, nitrate, nitrite, phosphates, and electrical conductivity were important factors affecting MXR activity.