Preparation of chitosan water-in-oil emulsions by stirred cell membrane emulsification

In order to provide a method of low-energy membrane emulsification process in food and pharmaceutical industries the water-in-oil (W/O) chitosan emulsions with a narrow droplet size distribution were prepared using 0.1 & mu;m pore size polyethylene terephthalate (PET) track-etched membranes. Droplet size distributions and emulsion stability were studied by light scattering analysis. The effects of emulsifier concentration, rotation speed, and chitosan concentration on droplet size and stability of emulsions were evaluated. As a result, a monodispersed pH responsive emulsion with droplet size of 300 & PLUSMN; 40 nm was prepared. The release of Vitamin B5 (calcium pantothenate) from the prepared emulsion was studied at various temperatures and pH. The temperature increase contributes to the faster release of an active compound from chitosan emulsion. According to the obtained results, the rate of calcium pantothenate release reduced with an increase of pH value. Moreover, the mechanism of diffusion was changed. The results of this research present a roadmap for future applications of membrane emulsification and encapsulation of hydrophilic or hydrophobic active compounds in food, pharmaceutical, and cosmetic technology.

Automated summary

In this study, a low-energy membrane emulsification process was developed for the preparation of water-in-oil (W/O) chitosan emulsions with a narrow droplet size distribution. The effects of emulsifier concentration, rotation speed, and chitosan concentration on droplet size and stability of emulsions were evaluated. The prepared emulsion showed a monodispersed pH responsive behavior with a droplet size of 300 ± 40 nm. The release of Vitamin B5 (calcium pantothenate) from the emulsion was temperature and pH dependent.