Risk Factors, Temporal Dependence, and Seasonality of Human extended-spectrum beta-lactamases-producing Escherichia coli and Klebsiella pneumoniae Colonization in Malawi: A Longitudinal Model-based Approach

Water, sanitation, and hygiene (WASH) factors are important drivers of extended-spectrum beta-lactamases (ESBL)-producing Escherichia coli transmission, with contaminated water and/or inappropriate infrastructure the likely mode of acquisition. In contrast, antimicrobial exposure appears as the key driver for ESBL-producing Klebsiella pneumoniae. Background Sub-Saharan Africa has the highest estimated death rate attributable to antimicrobial resistance, especially from extended-spectrum beta-lactamase-producing Enterobacterales (ESBL-E). However, the dynamics of human colonization in the community with ESBL-E are not well described. Inadequate water, sanitation, and hygiene infrastructure and associated behaviors are believed to play an important role in transmission of ESBL-E, and an improved understanding of the temporal dynamics of within-household transmission could help inform the design of future policies. Methods In this 18-month study, using microbiological data and household surveys, we built a multivariable hierarchical harmonic logistic regression model to identify risk factors for colonization with ESBL-producing Escherichia coli and Klebsiella pneumoniae, reflecting household structure and temporal correlation of colonization status. Results Being male was associated with a lower risk of colonization with ESBL-producing E. coli (odds ratio [OR], 0.786; credible interval [CrI], .678-.910), whereas the use of a tube well or a borehole was associated with an increased risk (OR, 1.550; CrI, 1.003-2.394). For ESBL-producing K. pneumoniae, recent antibiotic exposure increased risk of colonization (OR, 1.281; CrI, 1.049-1.565), whereas sharing plates decreased that risk (OR, 0.672; CrI, .460-.980). Finally, the temporal correlation range of 8 to 11 weeks provided evidence that within-household transmission occurs within this time frame. Conclusions We describe different risks for colonization with different enteric bacterial species. Our findings suggest interventions to reduce transmission targeted at the household level need to focus on improving water, sanitation, and hygiene infrastructure and associated behaviors, whereas at the community level, they should focus on both environmental hygiene and antibiotic stewardship.

Automated summary

Water, sanitation, and hygiene (WASH) factors play a crucial role in the transmission of extended-spectrum beta-lactamases (ESBL)-producing Escherichia coli, while antimicrobial exposure is the key driver for ESBL-producing Klebsiella pneumoniae.