期刊
CLINICAL AND EXPERIMENTAL IMMUNOLOGY
卷 212, 期 3, 页码 285-295出版社
OXFORD UNIV PRESS
DOI: 10.1093/cei/uxad029
关键词
single-cell RNA sequence; endometriosis-associated infertility; endometrial immune cells; endometrial receptivity
类别
We systematically investigated the immune landscape of eutopic endometrial single cells in minimal/mild endometriosis and compared it with disease-free controls. Our study sheds light on the inflammatory microenvironment and impaired endometrial receptivity in patients with endometriosis-associated infertility. The findings provide insights into the immunopathogenesis of endometriosis-associated infertility.
We systematically revealed the eutopic endometrial single-cell transcriptome immune landscape in minimal/mild endometriosis and compared it with that of disease-free controls. Our study provides new insights into the eutopic endometrial inflammatory microenvironment and defective endometrial receptivity in patients with endometriosis-associated infertility. Our results give clues into immunopathogenesis of endometriosis endometriosis-associated infertility. Endometriosis is a common inflammatory disorder in women of reproductive age due to an abnormal endometrial immune environment and is associated with infertility. This study aimed to systematically understand the endometrial leukocyte types, inflammatory environment, and impaired receptivity at single-cell resolution. We profiled single-cell RNA transcriptomes of 138 057 endometrial cells from endometriosis patients (n = 6) and control (n = 7), respectively, using 10x Genomics platform. We found that one cluster of epithelial cells that expressed PAEP and CXCL14 was mostly from the control during the window of implantation (WOI). This epithelial cell type is absent in the eutopic endometrium during the secretory phase. The proportion of endometrial immune cells decreased in the secretory phase in the control group, whereas the cycle variation of total immune cells, NK cells, and T cells was absent in endometriosis. Endometrial immune cells secreted more IL-10 in the secretory phase than in the proliferative phase in the control group; the opposite trend was observed in endometriosis. Proinflammatory cytokines levels in the endometrial immune cells were higher in endometriosis than in the control group. Trajectory analysis revealed that the secretory phase epithelial cells decreased in endometriosis. Ligand-receptor analysis revealed that 11 ligand-receptor pairs were upregulated between endometrial immune and epithelial cells during WOI. These results provide new insights into the endometrial immune microenvironment and impaired endometrial receptivity in infertile women with minimal/mild endometriosis.
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