Hantzsch Ester as Efficient and Economical NAD(P)H Mimic for In Vitro Bioredox Reactions

Biocatalysis has emerged as a valuable and reliable tool for industrial and academic societies, particularly in fields related to bioredox reactions. The cost of cofactors, especially those needed to be replenished at stoichiometric amounts or more, is the chief economic concern for bioredox reactions. In this study, a readily accessible, inexpensive, and bench-stable Hantzsch ester is verified as the viable and efficient NAD(P)H mimic by four enzymatic redox transformations, including two non-heme diiron N-oxygenases and two flavin-dependent reductases. This finding provides the potential to significantly reduce the costs of NAD(P)H-relying bioredox reactions.

Automated summary

Biocatalysis is a valuable tool for industrial and academic societies, especially in the field of bioredox reactions. The cost of cofactors, especially those that need to be replenished in stoichiometric amounts, is a major concern. This study verifies a readily accessible, inexpensive, and stable Hantzsch ester as an efficient NAD(P)H mimic, which can significantly reduce the costs of NAD(P)H-relying bioredox reactions.