期刊
CHEMISTRY-A EUROPEAN JOURNAL
卷 -, 期 -, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.202301113
关键词
CO2 reduction; formate dehydrogenase; immobilization; magnetic ZIF-8; mild conditions
A recyclable bio-composed system was constructed by encapsulating formate dehydrogenase in a ZIF-8 metalorganic framework (MOF) with the presence of magnetite, which overcomes the limitations of enzyme denaturation and the impossibility of biocatalyst recovery. The partial dissolution of ZIF-8 in the enzyme's operation medium can be inhibited by using a magnetic support concentration exceeding 10 mg mL(-1). The bio-friendly immobilization environment does not harm the integrity of the biocatalyst and improves the production of formic acid 3.4-fold compared to the free enzyme due to the concentration effect of MOFs as enzymatic cofactor concentrators. Furthermore, the bio-composed system retains 86% of its activity after five cycles, indicating excellent magnetic recovery and reusability.
The enzymatic reduction of carbon dioxide presents limited applicability due to denaturation and the impossibility of biocatalyst recovery; disadvantages that can be minimized by its immobilization. Here, a recyclable bio-composed system was constructed by in-situ encapsulation under mild conditions using formate dehydrogenase in a ZIF-8 metalorganic framework (MOF) in the presence of magnetite. The partial dissolution of ZIF-8 in the enzyme's operation medium can be relatively inhibited if the concentration of magnetic support used exceeds 10 mg mL(-1). The bio-friendly environment for immobilization does not harm the integrity of the biocatalyst, and the production of formic acid is improved 3.4-fold compared to the free enzyme because the MOFs act as concentrators of the enzymatic cofactor. Furthermore, the bio-composed system retains 86 % of its activity after a long time of five cycles, thus indicating an excellent magnetic recovery and a good reusability.
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