Natural Geranylated Sulfur-Containing Amides with Inhibitory Effect on Th17 Differentiation

Chemical investigation of medicinal plant Glycosmis lucida Wall. ex C. C. Huang leaves led to the production of ten compounds (1-10), including two previously unreported geranylated sulfurcontaining amides (1 and 2) and eight known ones (3-10). Structural characterization was carried out using comprehensive spectroscopic methods including NMR, MS and CD. The inhibitory effects of all isolates on Th17 differentiation were evaluated, of which compounds 1 and 6 significantly inhibited Th17 differentiation with IC50 values of 0.36 and 1.30 mu M, respectively, while both 1 and 6 failed to bind to retinoic acid-related orphan receptor gamma t (ROR gamma t), suggesting that their inhibition of Th17 differentiation is independent of ROR gamma t.

Automated summary

Chemical investigation of Glycosmis lucida Wall. ex C. C. Huang leaves led to the isolation of ten compounds, including two previously unreported geranylated sulfur-containing amides (1 and 2) and eight known compounds (3-10). Structural characterization was conducted using NMR, MS, and CD spectroscopic methods. Evaluation of the inhibitory effects of the isolates on Th17 differentiation revealed that compounds 1 and 6 significantly inhibited Th17 differentiation, with IC50 values of 0.36 and 1.30 μM, respectively. However, both compounds 1 and 6 failed to bind to retinoic acid-related orphan receptor gamma t (RORγt), indicating that their inhibition of Th17 differentiation is independent of RORγt.