4.4 Article

Facile Production of the Pseudomonas aeruginosa Virulence Factor LasB in Escherichia coli for Structure-Based Drug Design

相关参考文献

注意:仅列出部分参考文献,下载原文获取全部文献信息。
Article Chemistry, Medicinal

Chemical Optimization of Selective Pseudomonas aeruginosa LasB Elastase Inhibitors and Their Impact on LasB-Mediated Activation of IL-1β in Cellular and Animal Infection Models

Martin J. Everett et al.

Summary: This study describes the chemical optimization of specific LasB inhibitors and their impact in cellular and animal models of P. aeruginosa infection. Competitive inhibition of LasB was demonstrated through structural and kinetic studies. The highly potent and soluble LasB inhibitors show clear potential for the clinical development of a novel therapy for life-threatening respiratory infections caused by P. aeruginosa.

ACS INFECTIOUS DISEASES (2023)

Review Infectious Diseases

The Structures and Binding Modes of Small-Molecule Inhibitors of Pseudomonas aeruginosa Elastase LasB

Virgyl Camberlein et al.

Summary: LasB, a zinc metalloprotease and a crucial virulence factor of Pseudomonas aeruginosa, has become a key target in the development of novel antivirulence agents. This review provides an overview of the structure of its active site and a summary of the disclosed P. aeruginosa LasB inhibitors, with a specific focus on their binding modes and strategies for targeting LasB by small molecules.

ANTIBIOTICS-BASEL (2022)

Review Microbiology

Pseudomonas aeruginosa reference strains PAO1 and PA14: A genomic, phenotypic, and therapeutic review

Amber Grace et al.

Summary: Pseudomonas aeruginosa is a common pathogen that has been identified as a multi-drug resistant strain. PAO1 and PA14, two laboratory strains, have contributed significantly to our understanding of the bacterium. However, there are discrepancies between their behaviors and therapeutic susceptibilities observed in human infections. This review aims to compare the two strains and propose methods to improve their clinical relevance.

FRONTIERS IN MICROBIOLOGY (2022)

Article Chemistry, Medicinal

Phosphonate as a Stable Zinc-Binding Group for Pathoblocker Inhibitors of Clostridial Collagenase H (ColH)

Katrin Voos et al.

Summary: Microbial infections pose a significant threat to public health, and the rise in resistance calls for urgently needed new antibiotics. The zinc metalloprotease collagenase H (ColH) from Clostridium histolyticum is a virulence factor that catalyses tissue damage, making it an attractive target for new antivirulence agents. A phosphonate motif as a zinc-binding group showed promising activity against ColH, improved selectivity, and higher stability, making it a promising candidate for future drug development.

CHEMMEDCHEM (2021)

Review Pharmacology & Pharmacy

Pseudomonas aeruginosa elastase (LasB) as a therapeutic target

Martin Everett et al.

Summary: The Pseudomonas aeruginosa LasB elastase is a key protease in the pathogen's secretome, involved in host protein degradation and immune response disruption. Developing specific LasB inhibitors could be a promising approach for the treatment of P. aeruginosa infections.

DRUG DISCOVERY TODAY (2021)

Article Microbiology

Type 3 secretion system of Pseudomonas aeruginosa

Gertrudis Horna et al.

Summary: Pseudomonas aeruginosa is an opportunistic pathogen that mainly affects severe patients in ICUs, characterized by high levels of antibiotic resistance and a long battery of virulence factors. Among these factors, T3SS plays a key role in injecting effectors into host cells and influencing immune responses to increase bacterial survival rates. Despite extensive research on T3SS, there are still questions and further points to be clarified or established. Efforts are being made to develop T3SS-targeting molecules to improve patient outcomes.

MICROBIOLOGICAL RESEARCH (2021)

Review Biochemistry & Molecular Biology

Pseudomonas aeruginosa Biofilms

Minh Tam Tran Thi et al.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2020)

Article Biochemistry & Molecular Biology

The EMBL-EBI search and sequence analysis tools APIs in 2019

Fabio Madeira et al.

NUCLEIC ACIDS RESEARCH (2019)

Article Biochemical Research Methods

High-level expression of pseudolysin, the extracellular elastase of Pseudomonas aeruginosa, in Escherichia coli and its purification

Odutayo O. Odunuga et al.

PROTEIN EXPRESSION AND PURIFICATION (2015)

Review Cell Biology

The hierarchy quorum sensing network in Pseudomonas aeruginosa

Jasmine Lee et al.

PROTEIN & CELL (2015)

Review Microbiology

Targeting virulence: can we make evolution-proof drugs?

Richard C. Allen et al.

NATURE REVIEWS MICROBIOLOGY (2014)

Article Biochemical Research Methods

Features and development of Coot

P. Emsley et al.

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY (2010)

Article Biochemical Research Methods

PHENIX: a comprehensive Python-based system for macromolecular structure solution

Paul D. Adams et al.

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY (2010)

Article Biochemistry & Molecular Biology

Functional study of elafin cleaved by Pseudomonas aeruginosa metalloproteinases

Nicolas Guyot et al.

BIOLOGICAL CHEMISTRY (2010)

Article Respiratory System

Pseudomonas aeruginosa lasR mutants are associated with cystic fibrosis lung disease progression

Lucas R. Hoffman et al.

JOURNAL OF CYSTIC FIBROSIS (2009)

Article Chemistry, Multidisciplinary

Phaser crystallographic software

Airlie J. McCoy et al.

JOURNAL OF APPLIED CRYSTALLOGRAPHY (2007)

Review Biochemistry & Molecular Biology

Targeting virulence: a new paradigm for antimicrobial therapy

Anne E. Clatworthy et al.

NATURE CHEMICAL BIOLOGY (2007)

Article Biochemistry & Molecular Biology

Maturation of Pseudomonas aeruginosa elastase -: Formation of the disulfide bonds

P Braun et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2001)