期刊
CELL BIOLOGY INTERNATIONAL
卷 47, 期 7, 页码 1247-1258出版社
WILEY
DOI: 10.1002/cbin.12017
关键词
apoptosis; caspase activation; K562; megakaryocyte; myeloid differentiation; ormeloxifene
类别
ORM can induce megakaryocytic and myeloid differentiation in the K562 cell line. This induction is mediated by activation of ERK and induction of autophagy. Additionally, inhibition of caspases can mitigate megakaryocytic differentiation. These findings suggest that ORM has potential as an inducer of megakaryocytic differentiation.
Ormeloxifene (ORM) (3,4-trans-2,2-dimethyl-3-phenyl-4-p-(beta-pyrrolidinoethoxy) phenyl-7-methoxychroman), world's first nonsteroidal selective estrogen receptor modulator approved for contraception in India has been shown to have potential anticancer activities. Here, we show that ORM can induce megakaryocyte and myeloid (granulocytic) but not erythroid differentiation in multipotent human myeloid leukemia cell line K562. We show that ORM at an IC50 of 7.5 mu M can induce morphological changes similar to megakaryocytes in K562 cells. ORM led to increase in levels of megakaryocytic differentiation markers (CD41 and CD61) as well as key transcription factors GATA1 and AML1. We further show that ORM induces megakaryocytic differentiation in K562 cells through ERK activation and induction of autophagy in a fashion similar to other known inducers of megakaryocytic differentiation such as phorbol esters. In addition, as shown earlier, we yet again observed that ORM led to activation of caspases since their inhibition through pan-caspase inhibitor mitigated megakaryocytic differentiation as they led to significant decrease in CD41 and CD61. Because induction of megakaryocytic differentiation in K562 involves growth arrest and exit from cell cycle, we also observed an increase in levels of p21 and p27 with decrease in c-Myc protein levels in K562 cells treated with 7.5 mu M ORM for 24 and 48 h, respectively. Taken together, these findings indicate that ORM can markedly induce megakaryocytic differentiation in K562 cells.
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