Genetic mapping and identification of Rht8-B1 that regulates plant height in wheat

BackgroundPlant height (PH) and spike compactness (SC) are important agronomic traits that affect yield improvement in wheat crops. The identification of the loci or genes responsible for these traits is thus of great importance for marker-assisted selection in wheat breeding. ResultsIn this study, we used a recombinant inbred line (RIL) population with 139 lines derived from a cross between the mutant Rht8-2 and the local wheat variety NongDa5181 (ND5181) to construct a high-density genetic linkage map by applying the Wheat 40 K Panel. We identified seven stable QTLs for PH (three) and SC (four) in two environments using the RIL population, and found that Rht8-B1 is the causal gene of qPH2B.1 by further genetic mapping, gene cloning and gene editing analyses. Our results also showed that two natural variants from GC to TT in the coding region of Rht8-B1 resulted in an amino acid change from G (ND5181) to V (Rht8-2) at the 175(th) position, reducing PH by 3.6%similar to 6.2% in the RIL population. Moreover, gene editing analysis suggested that the height of T-2 generation in Rht8-B1 edited plants was reduced by 5.6%, and that the impact of Rht8-B1 on PH was significantly lower than Rht8-D1. Additionally, analysis of the distribution of Rht8-B1 in various wheat resources suggested that the Rht8-B1b allele has not been widely utilized in modern wheat breeding. ConclusionsThe combination of Rht8-B1b with other favorable Rht genes might be an alternative approach for developing lodging-resistant crops. Our study provides important information for marker-assisted selection in wheat breeding.

Automated summary

In this study, a high-density genetic linkage map was constructed using a recombinant inbred line (RIL) population derived from a mutant Rht8-2 and a local wheat variety. Seven stable QTLs for plant height and spike compactness were identified, and Rht8-B1 was confirmed as the causal gene for qPH2B.1. Furthermore, it was found that Rht8-B1 had a lower impact on plant height compared to Rht8-D1. These findings provide important information for marker-assisted selection in wheat breeding.