期刊
BIOTECHNOLOGY PROGRESS
卷 -, 期 -, 页码 -出版社
WILEY
DOI: 10.1002/btpr.3369
关键词
Box-Behnken design; carotenoids; Plackett-Burman design; response surface methods; Umbelopsis ramanniana
In this study, Umbelopsis ramanniana was investigated to enhance carotenoid production. Various carbon and nitrogen sources were evaluated, with KNO3 and lactose being the most effective nitrogen and carbon sources, respectively. The optimization of medium components was achieved using Plackett-Burman design, and Box-Behnken response surface methodology was applied to further optimize carotenoid and biomass production. The optimum conditions for maximum carotenoid and biomass production were determined as a lactose concentration of 32.42 g/L, a carbon to nitrogen ratio of 20:1, and a shaking speed of 130 rpm, resulting in a significant increase in carotenoid and biomass production.
Umbelopsis ramanniana was investigated to increase carotenoid production. Nine different carbon sources and six different nitrogen sources were evaluated for the maximum carotenoid production. The most effective nitrogen and carbon sources were KNO3 and lactose, respectively. Then, the optimization of medium components for enhancement of carotenoid production by Umbelopsis ramanniana was achieved using Plackett-Burman design. Box-Behnken response surface methodology was applied to further optimize carotenoid and biomass production. Carbon to nitrogen ratio, lactose concentration, and shaking speed were studied as variables in Box-Behnken design. The optimum conditions for carotenoid and biomass production were determined as 32.42 g/L of lactose concentration, 20:1 of carbon to nitrogen ratio, and shaking speed of 130 rpm. The maximum carotenoid and biomass production under optimized conditions were 1141 mu g/L (beta-carotene-Eq) and 13.14 g/L, respectively. When compared to the control fermentation, carotenoid, and biomass production were increased by about 2 and 1.3 folds, respectively.
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