期刊
BIOTECHNOLOGY AND BIOENGINEERING
卷 -, 期 -, 页码 -出版社
WILEY
DOI: 10.1002/bit.28432
关键词
coexpression; d-amino acid aminotransferase; d; l-phosphinothricin; enzyme cascade; 2-oxo-4 [(hydroxy)(methyl)phosphinoyl]butyric acid
This study developed a biocatalytic cascade for the production of PPO with high efficiency and low cost. The cascade integrated different enzymes and showed superior catalytic efficiency in the synthesis of PPO from d,l-PPT. It achieved high space-time yield and complete conversion of the substrate.
2-oxo-4-[(hydroxy)(methyl)phosphinoyl]butyric acid (PPO) is the essential precursor keto acid for the asymmetric biosynthesis of herbicide l-phosphinothricin (l-PPT). Developing a biocatalytic cascade for PPO production with high efficiency and low cost is highly desired. Herein, a d-amino acid aminotransferase from Bacillus sp. YM-1 (Ym DAAT) with high activity (48.95 U/mg) and affinity (K-m = 27.49 mM) toward d-PPT was evaluated. To circumvent the inhibition of by-product d-glutamate (d-Glu), an amino acceptor (alpha-ketoglutarate) regeneration cascade was constructed as a recombinant Escherichia coli (E. coli D), by coupling Ym d-AAT, d-aspartate oxidase from Thermomyces dupontii (TdDDO) and catalase from Geobacillus sp. CHB1. Moreover, the regulation of the ribosome binding site was employed to overcome the limiting step of expression toxic protein TdDDO in E. coli BL21(DE3). The aminotransferase-driven whole-cell biocatalytic cascade (E. coli D) showed superior catalytic efficiency for the synthesis of PPO from d,l-phosphinothricin (d,l-PPT). It revealed the production of PPO exhibited high space-time yield (2.59 g L-1 h(-1)) with complete conversion of d-PPT to PPO at high substrate concentration (600 mM d,l-PPT) in 1.5 L reaction system. This study first provides the synthesis of PPO from d,l-PPT employing an aminotransferase-driven biocatalytic cascade.
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