Alternative biological sources for extracellular vesicles production and purification strategies for process scale-up

Article Engineering, Biomedical

Plant-derived nanovesicles: Current understanding and applications for cancer therapy

Ngoc Phung Ly et al.

Summary: This article provides a comprehensive introduction to plant-derived vesicles (PDVs), including their biological characteristics, purification methods, and a summary of their current development potential for cancer therapy.

BIOACTIVE MATERIALS (2023)

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Article Biochemical Research Methods

GFP-tagging of extracellular vesicles for rapid process development

Braulio Carrillo Sanchez et al.

Summary: Extracellular vesicles (EVs) are considered as nano-scale molecular messengers due to their ability to deliver bioactive cargos. This study presents the use of GFP-tagged EVs for rapid quantification and monitoring of EVs production, highlighting the utility of this approach for accelerated process development.

BIOTECHNOLOGY JOURNAL (2022)

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Article Biochemical Research Methods

Investigating heparin affinity chromatography for extracellular vesicle purification and fractionation

Benjamin Barnes et al.

Summary: The purification of extracellular vesicles (EVs) is a major challenge in fundamental research and commercial applications. The study investigates the potential of heparin affinity chromatography (HAC) in purifying neural stem cell-derived EVs. HAC demonstrates high efficiency in removing residual protein and DNA, and it can also separate EVs into different subgroups.

JOURNAL OF CHROMATOGRAPHY A (2022)

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Article Cell Biology

Distinguishing functional exosomes and other extracellular vesicles as a nucleic acid cargo by the anion-exchange method

Naohiro Seo et al.

Summary: The study proposes an effective method for preparing high-performance exosomes (EXOs) using an anion-exchange method. This method allows for the large-scale separation of bioactive EXOs and MV-like EVs, providing valuable insights for research on reliable bioactivity and drug discovery of extracellular vesicles.

JOURNAL OF EXTRACELLULAR VESICLES (2022)

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Review Biochemical Research Methods

Affinity-based isolation of extracellular vesicles and the effects on downstream molecular analysis

Gisela Stroehle et al.

Summary: Extracellular vesicles (EVs) are transport vesicles secreted by cells, and their isolation through affinity-based methods can affect downstream analysis of EV molecular cargo.

ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2022)

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Review Pharmacology & Pharmacy

Scaled preparation of extracellular vesicles from conditioned media

Simon Staubach et al.

Summary: Extracellular vesicles (EVs), especially those from mesenchymal stem/stromal cells (MSCs), are being increasingly explored as biotherapeutic agents for various diseases. To translate them effectively into clinical applications, scalable production processes that comply with good manufacturing practice (GMP) are necessary. The scalability of conventional EV preparation methods poses a major challenge in the therapeutic EV field, requiring the identification of appropriate concentration and purification methods for scale-up. Adopting principles from enveloped viruses, which have been used in clinics for over two decades, can provide valuable insights for EV manufacturing.

ADVANCED DRUG DELIVERY REVIEWS (2021)

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Article Engineering, Environmental

Micro fluidics for extracellular vesicle separation and mimetic synthesis: Recent advances and future perspectives

Yingchao Meng et al.

Summary: Microfluidic technologies are gaining interest for their potential in fast and efficient extracellular vesicle processing, highlighting recent advances in EV separation and mimetics synthesis for drug delivery applications. The discussion also touches on the advantages and drawbacks of current technologies, as well as addressing future challenges and applications in the field.

CHEMICAL ENGINEERING JOURNAL (2021)

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Article Biochemical Research Methods

Modern isolation and separation techniques for extracellular vesicles

Thanaporn Liangsupree et al.

Summary: Extracellular vesicles (EVs) are crucial in cellular communication and regulating physiological and pathological processes. Modern isolation techniques are characterized by being time-efficient, easy to operate, and capable of providing EVs with suitable purity and integrity for various applications.

JOURNAL OF CHROMATOGRAPHY A (2021)

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Review Biotechnology & Applied Microbiology

Plant Exosome-like Nanovesicles: Emerging Therapeutics and Drug Delivery Nanoplatforms

Haseeb Anwar Dad et al.

Summary: Plant exosome-like nanovesicles, rich in bioactive components, hold great potential in the fields of biotreatment and drug delivery, exhibiting immunomodulatory, antitumor, regenerative, and anti-inflammatory effects. Their physicochemical traits make them promising candidates for next-generation therapeutic and drug delivery nanoplatforms.

MOLECULAR THERAPY (2021)

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Article Biochemistry & Molecular Biology

Strawberry-Derived Exosome-Like Nanoparticles Prevent Oxidative Stress in Human Mesenchymal Stromal Cells

Francesca Perut et al.

Summary: EPDENs isolated from strawberries have been shown to have potential health-promoting activities, including preventing oxidative stress in human mesenchymal stromal cells. The presence of vitamin C in the EPDEN membrane may contribute to this effect, along with the small RNAs and miRNAs found in the cargo. These findings suggest that Fragaria-derived EPDENs may serve as nanoshuttles in food with bioactive properties.

BIOMOLECULES (2021)

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Article Agriculture, Dairy & Animal Science

Comparison of isolation methods using commercially available kits for obtaining extracellular vesicles from cow milk

Mai Morozumi et al.

Summary: The study aimed to compare methods for isolating milk-derived extracellular vesicles (EV), finding that acetic acid precipitation was the best method for removing casein. The SEC-based qEV column was able to collect purer and higher quantities of milk-derived EV.

JOURNAL OF DAIRY SCIENCE (2021)

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Review Nutrition & Dietetics

Potential Mechanisms Linking Food-Derived MicroRNAs, Gut Microbiota and Intestinal Barrier Functions in the Context of Nutrition and Human Health

Ester Diez-Sainz et al.

Summary: MiRNAs are small non-coding RNA molecules that play a crucial role in regulating gene expression. Dietary plant-derived miRNAs may be incorporated into humans through various mechanisms and have an impact on gut microbiota composition, intestinal barrier permeability, and overall health. The interaction between xenomiRs and gut microbiota could potentially explain the variation in research results regarding dietary miRNA uptake and function in humans.

FRONTIERS IN NUTRITION (2021)

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Article Cell Biology

Surface Marker Expression in Small and Medium/Large Mesenchymal Stromal Cell-Derived Extracellular Vesicles in Naive or Apoptotic Condition Using Orthogonal Techniques

Renata Skovronova et al.

Summary: The study demonstrates that extracellular vesicles released by mesenchymal stromal cells (MSC-EVs) are a promising resource for regenerative medicine, with potential applications in clinical settings. Analyzing the identity and purity of MSC-EVs can provide a safer profile for their clinical application.

CELLS (2021)

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Article Nutrition & Dietetics

Regular Industrial Processing of Bovine Milk Impacts the Integrity and Molecular Composition of Extracellular Vesicles

Marije Kleinjan et al.

Summary: Commercial milk subjected to pasteurization or UHT heating contains fewer or no intact EVs, respectively. Although most EVs appear resistant to pasteurization based on particle numbers, their integrity and molecular composition are altered. This may decrease or change the potential transfer of bioactive components through bovine milk EVs to human consumers in heat-treated commercial milk.

JOURNAL OF NUTRITION (2021)

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Article Multidisciplinary Sciences

Extracellular vesicles isolated from milk can improve gut barrier dysfunction induced by malnutrition

Mohamed Karim Maghraby et al.

Summary: The study revealed that EVs enriched from milk have the potential to improve intestinal barrier dysfunction caused by malnutrition and enhance cellular proliferation and intestinal structure restoration.

SCIENTIFIC REPORTS (2021)

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Review Microbiology

Microbiota-host communications: Bacterial extracellular vesicles as a common language

Rogers A. Nahui Palomino et al.

Summary: Both gram-negative and gram-positive bacteria release extracellular vesicles (EVs) that contain components from their mother cells. Bacterial EVs are believed to mediate bacteria-host communications and play important roles in bacteria-bacteria and bacteria-host interactions. The understanding of bacterial EVs may lead to new therapeutics and diagnostics.

PLOS PATHOGENS (2021)

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Article Cell Biology

Nanoalgosomes: Introducing extracellular vesicles produced by microalgae

Giorgia Adamo et al.

Summary: The study of extracellular vesicles (EVs) for cellular, inter-organismal and cross kingdom communication has high expectations for various bio-technological applications. Microalgae are sustainable and renewable sources of bioactive compounds with sectoral applications in health supplements, cosmetic products, and food ingredients. The newly discovered subtype of EVs derived from microalgae, nanoalgosomes, has shown potential for efficient uptake by mammalian cell lines, demonstrating the cross kingdom communication capabilities of EVs.

JOURNAL OF EXTRACELLULAR VESICLES (2021)

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Article Biochemistry & Molecular Biology

Preliminary Results of CitraVes™ Effects on Low Density Lipoprotein Cholesterol and Waist Circumference in Healthy Subjects after 12 Weeks: A Pilot Open-Label Study

Stefania Raimondo et al.

Summary: The study evaluated the effects of a new plant-based product on cardiovascular risk factors in healthy subjects, indicating its potential to improve metabolic risk factors.

METABOLITES (2021)

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Review Pharmacology & Pharmacy

Bacterial extracellular vesicles: Understanding biology promotes applications as nanopharmaceuticals

Leila Pourtalebi Jahromi et al.

Summary: Bacterial vesicles mediate communication between cells containing various biomolecules. They have the potential for designing diverse therapeutic and diagnostic methods, but technical limitations must be addressed for production, purification, and characterization.

ADVANCED DRUG DELIVERY REVIEWS (2021)

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Article Agriculture, Dairy & Animal Science

Isolation of extracellular vesicles from byproducts of cheesemaking by tangential flow filtration yields heterogeneous fractions of nanoparticles

Sonal Sukreet et al.

Summary: This study tested the hypothesis of enriching small EV from byproducts of cheesemaking using tangential flow filtration. The results showed differences in protein content and specific protein markers between EV purified via tangential flow filtration and ultracentrifugation.

JOURNAL OF DAIRY SCIENCE (2021)

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Article Biochemistry & Molecular Biology

Immunomodulatory roles and novel applications of bacterial membrane vesicles

William J. Gilmore et al.

Summary: The article summarizes the production mechanisms and functions of BMVs released by Gram-negative and Gram-positive bacteria, as well as their crucial roles in promoting bacterial survival, mediating pathogenesis, and regulating host immune responses.

MOLECULAR IMMUNOLOGY (2021)

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Review Nanoscience & Nanotechnology

Extracellular vesicles as a next-generation drug delivery platform

Inge Katrin Herrmann et al.

Summary: Extracellular vesicles play a crucial role in cell-to-cell communication and can serve as drug carriers with advantages over synthetic carriers. However, clinical translation of extracellular vesicle-based therapies remains challenging, requiring further research and development efforts.

NATURE NANOTECHNOLOGY (2021)

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Article Cell & Tissue Engineering

The Antioxidant Effect of Small Extracellular Vesicles Derived from Aloe vera Peels for Wound Healing

Min Kang Kim et al.

Summary: This study isolated EVs from Aloe vera peels and explored their antioxidant and wound healing potential. The results showed that A-EVs had good cytocompatibility, antioxidant activity, and enhanced migration ability of human skin cells. Furthermore, A-EVs activated the antioxidant defense mechanisms and promoted wound healing process through Nrf2 activation.

TISSUE ENGINEERING AND REGENERATIVE MEDICINE (2021)

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Review Biochemistry & Molecular Biology

Bioactives from Bee Products and Accompanying Extracellular Vesicles as Novel Bioactive Components for Wound Healing

Zeljka Persuric et al.

Summary: Recent research has focused on identifying health-promoting compounds in bee products such as honey, royal jelly, propolis, and bee pollen. Mass spectrometry techniques have revealed the diversity in chemical composition of bee products, influenced by factors like botanical sources and geographical origin. Extracellular vesicles from bee products show promise as a novel bioactive nutraceutical component, with potential applications in wound healing and tissue regeneration.

MOLECULES (2021)

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Article Materials Science, Biomaterials

Isolation of extracellular vesicles from microalgae: towards the production of sustainable and natural nanocarriers of bioactive compounds

Sabrina Picciotto et al.

Summary: Safe, efficient, and specific nano-delivery systems are crucial for current and emerging therapeutics, with novel bio-based nanotechnologies focusing on extracellular vesicles (EVs) emerging. This study demonstrates that microalgae are promising producers of EVs, with potential for efficient production and a wide range of applications. By analyzing nanostructures from eighteen microalgal species, seven promising EV-producing strains were identified, indicating the evolutionary conservation of EV production in microalgae.

BIOMATERIALS SCIENCE (2021)

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Article Engineering, Biomedical

Cyclic tangential flow filtration system for isolation of extracellular vesicles

Kimin Kim et al.

Summary: The study introduces a cyclic tangential flow filtration system that can better isolate EVs in the 30-200nm size range through dual cyclic filtration. By introducing a buffer-exchange process after filtration, the purification efficiency is improved. EVs isolated by the cyclic TFF system exhibit more abundant exosome markers and active EVs compared to conventional methods.

APL BIOENGINEERING (2021)

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Article Toxicology

Safety of bovine milk derived extracellular vesicles used for delivery of RNA therapeutics in zebrafish and mice

Akiko Matsuda et al.

JOURNAL OF APPLIED TOXICOLOGY (2020)

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Article Pharmacology & Pharmacy

Cow Milk and Intestinal Epithelial Cell-Derived Extracellular Vesicles as Systems for Enhancing Oral Drug Delivery

Greta Carobolante et al.

PHARMACEUTICS (2020)

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Article Pharmacology & Pharmacy

Using microfluidics for scalable manufacturing of nanomedicines from bench to GMP: A case study using protein-loaded liposomes

Cameron Webb et al.

INTERNATIONAL JOURNAL OF PHARMACEUTICS (2020)

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Article Multidisciplinary Sciences

A cost-effective polyethylene glycol-based method for the isolation of functional edible nanoparticles from ginger rhizomes

Sreeram Peringattu Kalarikkal et al.

SCIENTIFIC REPORTS (2020)

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Article Biotechnology & Applied Microbiology

An efficient method to isolate lemon derived extracellular vesicles for gastric cancer therapy

Meng Yang et al.

JOURNAL OF NANOBIOTECHNOLOGY (2020)

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Review Biotechnology & Applied Microbiology

Technologies and Standardization in Research on Extracellular Vesicles

Srujan Gandham et al.

TRENDS IN BIOTECHNOLOGY (2020)

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Review Biochemistry & Molecular Biology

Emerging role of bacterial extracellular vesicles in cancer

Antonios Chronopoulos et al.

ONCOGENE (2020)

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Article Cell Biology

Methods for Separation and Characterization of Extracellular Vesicles: Results of a Worldwide Survey Performed by the ISEV Rigor and Standardization Subcommittee

Felix Royo et al.

CELLS (2020)

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Article Food Science & Technology

Biomanufacturing of Tomato-Derived Nanovesicles

Ramesh Bokka et al.

FOODS (2020)

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Review Medicine, Research & Experimental

Extracellular vesicles: the next generation of biomarkers for liquid biopsy-based prostate cancer diagnosis

Bairen Pang et al.

THERANOSTICS (2020)

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Article Biochemical Research Methods

Asymmetric-flow field-flow fractionation technology for exomere and small extracellular vesicle separation and characterization

Haiying Zhang et al.

NATURE PROTOCOLS (2019)

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Article Chemistry, Multidisciplinary

Plant-Derived Edible Nanoparticles and miRNAs: Emerging Frontier for Therapeutics and Targeted Drug-Delivery

Siavash Iravani et al.

ACS SUSTAINABLE CHEMISTRY & ENGINEERING (2019)

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Review Biochemical Research Methods

Scalable Production and Isolation of Extracellular Vesicles: Available Sources and Lessons from Current Industrial Bioprocesses

Carolina Paganini et al.

BIOTECHNOLOGY JOURNAL (2019)

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Article Medicine, Research & Experimental

Exosome-like Nanoparticles from Ginger Rhizomes Inhibited NLRP3 Inflammasome Activation

Xingyi Chen et al.

MOLECULAR PHARMACEUTICS (2019)

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Review Cell Biology

Advances in therapeutic applications of extracellular vesicles

Oscar P. B. Wiklander et al.

SCIENCE TRANSLATIONAL MEDICINE (2019)

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Review Biochemistry & Molecular Biology

The Role of Extracellular Vesicles in Cutaneous Remodeling and Hair Follicle Dynamics

Elisa Carrasco et al.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2019)

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Review Cell Biology

Overview of Extracellular Vesicles, Their Origin, Composition, Purpose, and Methods for Exosome Isolation and Analysis

Laura M. Doyle et al.

CELLS (2019)

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Article Cell Biology

A novel population of extracellular vesicles smaller than exosomes promotes cell proliferation

Sang-Soo Lee et al.

CELL COMMUNICATION AND SIGNALING (2019)

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Article Pharmacology & Pharmacy

Bacterial membrane vesicles as promising vaccine candidates

Linglei Jiang et al.

EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS (2019)

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Review Cell Biology

A review on protein markers of exosome from different bio-resources and the antibodies used for characterization

Fengyan Deng et al.

JOURNAL OF HISTOTECHNOLOGY (2019)

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Article Immunology

Spontaneously released Neisseria meningitidis outer membrane vesicles as vaccine platform: production and purification

Matthias J. H. Gerritzen et al.

VACCINE (2019)

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Article Materials Science, Multidisciplinary

Tangential Flow Microfluidics for the Capture and Release of Nanoparticles and Extracellular Vesicles on Conventional and Ultrathin Membranes

Mehdi Dehghani et al.

ADVANCED MATERIALS TECHNOLOGIES (2019)

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Article Biochemistry & Molecular Biology

In Vitro Wound Healing Activity of Wheat-Derived Nanovesicles

Fikrettin Sahin et al.

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY (2019)

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Article Microbiology

A Phytophthora Effector Suppresses Trans-Kingdom RNAi to Promote Disease Susceptibility

Yingnan Hou et al.

CELL HOST & MICROBE (2019)

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Article Chemistry, Medicinal

The Potential of Exosomes From Cow Milk for Oral Delivery

Jamie L. Betker et al.

JOURNAL OF PHARMACEUTICAL SCIENCES (2019)

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Article Biochemistry & Molecular Biology

Membrane Transporters in Citrus clementina Fruit Juice-Derived Nanovesicles

Christopher Stanly et al.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2019)

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Review Biotechnology & Applied Microbiology

Recent Developments in Bioprocessing of Recombinant Proteins: Expression Hosts and Process Development

Nagesh K. Tripathi et al.

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY (2019)

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Review Biochemistry & Molecular Biology

Biological properties of plant-derived extracellular vesicles

Sophie Rome

FOOD & FUNCTION (2019)

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Review Food Science & Technology

Naturally Occurring Exosome Vesicles as Potential Delivery Vehicle for Bioactive Compounds

Precious Akuma et al.

FRONTIERS IN SUSTAINABLE FOOD SYSTEMS (2019)

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Review Plant Sciences

Extracellular vesicles as key mediators of plant-microbe interactions

Brian D. Rutter et al.

CURRENT OPINION IN PLANT BIOLOGY (2018)

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Article Biochemical Research Methods

Simultaneous isolation and preconcentration of exosomes by ion concentration polarization

Steven Marczak et al.

ELECTROPHORESIS (2018)

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Article Plant Sciences

Protein biocargo of citrus fruit-derived vesicles reveals heterogeneous transport and extracellular vesicle populations

Gabriella Pocsfalvi et al.

JOURNAL OF PLANT PHYSIOLOGY (2018)

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Review Chemistry, Multidisciplinary

Technical challenges of working with extracellular vesicles

Marcel I. Ramirez et al.

NANOSCALE (2018)

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Article Cell Biology

Identification of distinct nanoparticles and subsets of extracellular vesicles by asymmetric flow field-flow fractionation

Haiying Zhang et al.

NATURE CELL BIOLOGY (2018)

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Review Biochemistry & Molecular Biology

Manufacturing Exosomes: A Promising Therapeutic Platform

Ivano Luigi Colao et al.

TRENDS IN MOLECULAR MEDICINE (2018)

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Article Multidisciplinary Sciences

Rapid isolation and enrichment of extracellular vesicle preparations using anion exchange chromatography

Nikki Heath et al.

SCIENTIFIC REPORTS (2018)

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Review Biotechnology & Applied Microbiology

Isolation of Extracellular Vesicles: General Methodologies and Latest Trends

Maria Yu. Konoshenko et al.

BIOMED RESEARCH INTERNATIONAL (2018)

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Article Biotechnology & Applied Microbiology

Exosomes Produced from 3D Cultures of MSCs by Tangential Flow Filtration Show Higher Yield and Improved Activity

Reka Agnes Haraszti et al.

MOLECULAR THERAPY (2018)

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Article Multidisciplinary Sciences

Milk exosomes are bioavailable and distinct microRNA cargos have unique tissue distribution patterns

Sonia Manca et al.

SCIENTIFIC REPORTS (2018)

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Article Microbiology

Plant-Derived Exosomal MicroRNAs Shape the Gut Microbiota

Yun Teng et al.

CELL HOST & MICROBE (2018)

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Review Pathology

Bacterial outer membrane vesicles, a potential vaccine candidate in interactions with host cells based

Wei Cai et al.

DIAGNOSTIC PATHOLOGY (2018)

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Article Medicine, Research & Experimental

Apple-Derived Nanoparticles Modulate Expression of Organic-Anion-Transporting Polypeptide (OATP) 261 in Caco-2 Cells

Daichi Fujita et al.

MOLECULAR PHARMACEUTICS (2018)

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Article Cell & Tissue Engineering

Large-Scale Preparation of Extracellular Vesicles Enriched with Specific microRNA

Kyung Whan Yoo et al.

TISSUE ENGINEERING PART C-METHODS (2018)

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Article Cell Biology

Tangential Flow Filtration for Highly Efficient Concentration of Extracellular Vesicles from Large Volumes of Fluid

Sara Busatto et al.

CELLS (2018)

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Article Cell Biology

Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines

Clotilde Thery et al.

JOURNAL OF EXTRACELLULAR VESICLES (2018)

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Article Cell Biology

Biocompatibility of highly purified bovine milk-derived extracellular vesicles

Masaharu Somiya et al.

JOURNAL OF EXTRACELLULAR VESICLES (2018)

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Review Cell Biology

Therapeutic biomaterials based on extracellular vesicles: classification of bio-engineering and mimetic preparation routes

Pablo Garcia-Manrique et al.

JOURNAL OF EXTRACELLULAR VESICLES (2018)

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Article Biotechnology & Applied Microbiology

Broccoli-Derived Nanoparticle Inhibits Mouse Colitis by Activating Dendritic Cell AMP-Activated Protein Kinase

Zhongbin Deng et al.

MOLECULAR THERAPY (2017)

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Article Microbiology

Affinity purification of bacterial outer membrane vesicles (OMVs) utilizing a His-tag mutant

Nathan J. Alves et al.

RESEARCH IN MICROBIOLOGY (2017)

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Article Multidisciplinary Sciences

Formation and purification of tailored liposomes for drug delivery using a module-based micro continuous-flow system

Nikolay Dimov et al.

SCIENTIFIC REPORTS (2017)

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Article Multidisciplinary Sciences

Escherichia coli outer membrane vesicles can contribute to sepsis induced cardiac dysfunction

Kristina Svennerholm et al.

SCIENTIFIC REPORTS (2017)

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Article Biochemistry & Molecular Biology

Curcumin Encapsulated in Milk Exosomes Resists Human Digestion and Possesses Enhanced Intestinal Permeability in Vitro

Monika Vashisht et al.

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY (2017)

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Article Chemistry, Multidisciplinary

The Exosome Total Isolation Chip

Fei Liu et al.

ACS NANO (2017)

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Review Biotechnology & Applied Microbiology

Bioengineering bacterial outer membrane vesicles as vaccine platform

Matthias J. H. Gerritzen et al.

BIOTECHNOLOGY ADVANCES (2017)

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Review Biotechnology & Applied Microbiology

Very-large-scale production of antibodies in plants: The biologization of manufacturing

J. F. Buyel et al.

BIOTECHNOLOGY ADVANCES (2017)

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Article Engineering, Biomedical

Edible ginger-derived nanoparticles: A novel therapeutic approach for the prevention and treatment of inflammatory bowel disease and colitis-associated cancer

Mingzhen Zhang et al.

BIOMATERIALS (2016)

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Article Oncology

Bovine milk-derived exosomes for drug delivery

Radha Munagala et al.

CANCER LETTERS (2016)

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Article Biochemistry & Molecular Biology

Extracellular Vesicles from Trypanosoma brucei Mediate Virulence Factor Transfer and Cause Host Anemia

Anthony J. Szempruch et al.

CELL (2016)

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Review Medicine, Research & Experimental

Regulation of chronic inflammatory and immune processes by extracellular vesicles

Paul D. Robbins et al.

JOURNAL OF CLINICAL INVESTIGATION (2016)

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Article Biotechnology & Applied Microbiology

Edible Ginger-derived Nano-lipids Loaded with Doxorubicin as a Novel Drug-delivery Approach for Colon Cancer Therapy

Mingzhen Zhang et al.

MOLECULAR THERAPY (2016)

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Article Immunology

Improving the immunogenicity of a trivalent Neisseria meningitidis native outer membrane vesicle vaccine by genetic modification

Lan Zhang et al.

VACCINE (2016)

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Article Multidisciplinary Sciences

A novel affinity-based method for the isolation of highly purified extracellular vesicles

Wataru Nakai et al.

SCIENTIFIC REPORTS (2016)

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Review Microbiology

Outer-membrane vesicles from Gram-negative bacteria: biogenesis and functions

Carmen Schwechheimer et al.

NATURE REVIEWS MICROBIOLOGY (2015)

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Article Oncology

Citrus limon-derived nanovesicles inhibit cancer cell proliferation and suppress CML xenograft growth by inducing TRAIL-mediated cell death

Stefania Raimondo et al.

ONCOTARGET (2015)

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Article Cell Biology

Ginger-derived nanoparticles protect against alcohol-induced liver damage

Xiaoying Zhuang et al.

JOURNAL OF EXTRACELLULAR VESICLES (2015)

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Article Cell Biology

Optimized exosome isolation protocol for cell culture supernatant and human plasma

Richard J. Lobb et al.

JOURNAL OF EXTRACELLULAR VESICLES (2015)

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Article Biochemical Research Methods

Benchtop isolation and characterization of functional exosomes by sequential filtration

Mitja L. Heinemann et al.

JOURNAL OF CHROMATOGRAPHY A (2014)

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Article Food Science & Technology

Interspecies communication between plant and mouse gut host cells through edible plant derived exosome-like nanoparticles

Jingyao Mu et al.

MOLECULAR NUTRITION & FOOD RESEARCH (2014)

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Article Biotechnology & Applied Microbiology

Targeted Drug Delivery to Intestinal Macrophages by Bioactive Nanovesicles Released from Grapefruit

Baomei Wang et al.

MOLECULAR THERAPY (2014)

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Article Multidisciplinary Sciences

Xylella fastidiosa outer membrane vesicles modulate plant colonization by blocking attachment to surfaces

Michael Ionescu et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2014)

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Review Physiology

Urinary extracellular vesicles and the kidney: biomarkers and beyond

Mahdi Salih et al.

AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY (2014)

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Review Biochemistry & Molecular Biology

Current methods for the isolation of extracellular vesicles

Fatemeh Momen-Heravi et al.

BIOLOGICAL CHEMISTRY (2013)

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Article Biotechnology & Applied Microbiology

Grape Exosome-like Nanoparticles Induce Intestinal Stem Cells and Protect Mice From DSS-Induced Colitis

Songwen Ju et al.

MOLECULAR THERAPY (2013)

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Article Multidisciplinary Sciences

Delivery of therapeutic agents by nanoparticles made of grapefruit-derived lipids

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