Response characteristics of denitrifying bacteria and denitrifying functional genes to woody peat during pig manure composting

This study aimed to explore the impacts of adding different proportions of woody peat (WP) (0%(CK), 5%(T1), and 15%(T2)) on denitrification during composting. The results demonstrated that compared with CK, T1 and T2 increased the total Kjeldahl nitrogen content (8% and 14%, respectively) and reduced the nitrate nitrogen (7% and 23%) content after composting. After composting, the abundances of nirK and nirS decreased by 4-9% and 33-35% under T1 and T2, respectively. Adding 15% WP reduced the abundances of key denitrifying bacteria such as Pseudomonas, Pusillimonas, Achromobacter, and Rhizobiales by 5-90%. The main factors that affected denitrification genes were the carbon content, nitrogen form (nitrite nitrogen and ammonium nitrogen), and denitrifying bacteria community. In summary, adding 15% WP has the best ability to reduce nitrogen loss by decreasing the abundances of denitrifying bacteria and denitrifying functional genes, thereby improving the agricultural value of composting products.

Automated summary

This study aimed to investigate the impact of adding different proportions of woody peat (WP) on denitrification during composting. The results showed that adding 5% and 15% WP increased the total nitrogen content and reduced the nitrate nitrogen content. The abundances of denitrification genes and denitrifying bacteria were also decreased with the addition of WP.