Impacts of exogenous quorum sensing signal molecule-acylated homoserine lactones (AHLs) with different addition modes on Anammox process

Anammox was proved having the quorum sensing ability, and several acylated homoserine lactones (AHLs) signal molecules were detected in the system. In this study, the impact of exogenous N-dodecanoyl homoserine lactone (C12-HSL) with different addition modes on the nitrogen removal, key enzymes' activity, and microbial revolution were investigated in Anammox system. Results showed that once-addition of C12-HSL had no obvious impact on Anammox. Daily-addition with 40 nM slightly improved the TN removal from 71.1 % to 74.5 %, while 80 and 200 nM significantly decreased it to 62.7 % and 61.8 %, respectively. The enzyme activity of ammonia monooxygenase increased from 0.015 to 0.068, nitrite reductase increased from 0.25 to 1.23, and nitrate reductase increased from 0.05 to 0.11 mu g NO2--N mg-1 Protein min-1. Arenimonas abundance showed positive correlation with TN removal while Candidatus Kuenenia was continuously suppressed. C12-HSL was beneficial for partial nitrification, and it could be adopted for regulating the nitrite production.

Automated summary

In this study, the impact of N-dodecanoyl homoserine lactone (C12-HSL) on nitrogen removal, enzyme activity, and microbial community in Anammox system was investigated. Results showed that once-addition of C12-HSL had no obvious impact on Anammox. Daily-addition with different concentrations of C12-HSL slightly improved or decreased the TN removal and enzyme activity. The abundance of certain bacteria showed correlation with TN removal.