A new hydrocyanine probe for imaging reactive oxygen species in the mitochondria of live cells

A new hydrocyanine probe containing a ROS-responsive element and a mitochondrial-targeting moiety, as well as a thiol-reactive chloromethyl group, has been designed for imaging reactive oxygen species (ROS) in the mitochondria of live cells. This hydrocyanine probe is cell-permeable, selectively targets mitochondria and responds to superoxide and hydroxyl radical, and can be used for detection of ROS production in the mitochondria of live cells caused by an oxidative stress.

Automated summary

A new hydrocyanine probe has been developed to image reactive oxygen species (ROS) in live cell mitochondria. This probe is cell-permeable, selectively targets mitochondria, and responds to superoxide and hydroxyl radical, allowing for detection of ROS production caused by oxidative stress in live cell mitochondria.