期刊
BIOMECHANICS AND MODELING IN MECHANOBIOLOGY
卷 -, 期 -, 页码 -出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s10237-022-01682-2
关键词
Cell migration; Cellular traction force; Substrate deformation; Strain energy density
Cells mechanically interact with their environment to sense various mechanical cues and this has profound effects on cellular behavior, including motility. This study aims to develop a mathematical model to predict the motility of individual cells in a colony on planar elastic substrates. The model takes into account cell-substrate interaction, substrate deformation from neighboring cells, and other factors such as friction, randomness, and cell death and division. The model can accurately simulate collective cell motility on planar elastic substrates and has the potential to be extended to other cell and substrate shapes and the inclusion of chemotactic cues.
Cells mechanically interact with their environment to sense, for example, topography, elasticity and mechanical cues from other cells. Mechano-sensing has profound effects on cellular behaviour, including motility. The current study aims to develop a mathematical model of cellular mechano-sensing on planar elastic substrates and demonstrate the model's predictive capabilities for the motility of individual cells in a colony. In the model, a cell is assumed to transmit an adhesion force, derived from a dynamic focal adhesion integrin density, that locally deforms a substrate, and to sense substrate deformation originating from neighbouring cells. The substrate deformation from multiple cells is expressed as total strain energy density with a spatially varying gradient. The magnitude and direction of the gradient at the cell location define the cell motion. Cell-substrate friction, partial motion randomness, and cell death and division are included. The substrate deformation by a single cell and the motility of two cells are presented for several substrate elasticities and thicknesses. The collective motility of 25 cells on a uniform substrate mimicking the closure of a circular wound of 200 mu m is predicted for deterministic and random motion. Cell motility on substrates with varying elasticity and thickness is explored for four cells and 15 cells, the latter again mimicking wound closure. Wound closure by 45 cells is used to demonstrate the simulation of cell death and division during migration. The mathematical model can adequately simulate the mechanically induced collective cell motility on planar elastic substrates. The model is suitable for extension to other cell and substrates shapes and the inclusion of chemotactic cues, offering the potential to complement in vitro and in vivo studies.
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