4.7 Article

Multisite Labeling of Proteins Using the Ligand-Directed Reactivity of Triggerable Michael Acceptors

相关参考文献

注意:仅列出部分参考文献,下载原文获取全部文献信息。
Review Chemistry, Multidisciplinary

Photoaffinity Labeling Chemistries Used to Map Biomolecular Interactions

Alexander V. West et al.

Summary: Photoaffinity labeling (PAL) is a biochemical technique that provides direct evidence of biomolecular interactions in cells. This review describes the major chemistries used in PAL experiments, discusses their advantages and disadvantages, and provides recommendations for designing a PAL probe based on recent examples.

ISRAEL JOURNAL OF CHEMISTRY (2023)

Article Chemistry, Multidisciplinary

Platform for Orthogonal N-Cysteine-Specific Protein Modification Enabled by Cyclopropenone Reagents

Alena Istrate et al.

Summary: In this study, a new protein conjugation strategy using CPO-based reagents for selective modification of N-terminal cysteine residues was reported. The method achieved stable linkage under mild conditions and was compatible with common reducing agent DTT.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2022)

Review Biochemistry & Molecular Biology

Chemical biology tools for imaging-based analysis of organelle membranes and lipids

Tomonori Tamura et al.

Summary: Membrane biology studies have shown that organelle membranes play a crucial role in addition to providing structural support. Various chemical biology tools have been developed to understand the functions, properties, and dynamics of organelle membranes. This review focuses on the latest molecular probes for organelle membrane studies, highlighting chemical strategies for visualizing and quantitatively analyzing the dynamic properties of organelle membranes and lipids, and discussing the current limitations and future directions of this challenging research area.

CURRENT OPINION IN CHEMICAL BIOLOGY (2022)

Article Multidisciplinary Sciences

Traceless cysteine-linchpin enables precision engineering of lysine in native proteins

Neelesh C. Reddy et al.

Summary: The authors report a method using cysteine-based chemoselective Linchpin Directed site-selective Modification of lysine residue in a protein, which provides high-level control over reactivity, chemoselectivity, site-selectivity, modularity, dual-probe installation, and protein-selectivity. The method offers protein selectivity by targeting a single lysine residue of a single protein in a complex biomolecular mixture, and it is useful for precision engineering of proteins.

NATURE COMMUNICATIONS (2022)

Article Chemistry, Multidisciplinary

Site-Specific Labeling of Endogenous Proteins Using CoLDR Chemistry

Rambabu N. Reddi et al.

Summary: Chemical modifications of proteins can impact various aspects of protein function, but there are limited methods for site-specific labeling in cells. The CoLDR strategy presented in this study allows for the installation of functional tags on target proteins while preserving their activity, enabling a wide range of applications. This approach, which includes labeling various proteins and assessing protein activity in a native environment, contributes to the tools available in chemical biology.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2021)

Article Chemistry, Multidisciplinary

Tunable Methacrylamides for Covalent Ligand Directed Release Chemistry

Rambabu N. Reddi et al.

Summary: This study introduces a new class of alpha-substituted methacrylamides as potential electrophiles for targeted covalent inhibitors, showing higher thiol reactivity and undergoing a conjugated addition-elimination reaction. These electrophiles demonstrated comparable potency and improved selectivity in various assays, making them a versatile addition to the toolbox of targeted covalent inhibitor design.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2021)

Review Biochemistry & Molecular Biology

Recent advances in identifying protein targets in drug discovery

Jaeyoung Ha et al.

Summary: Phenotype-based screening is a promising method for discovering first-in-class therapeutics, but understanding the mode of action has been a bottleneck for drug discovery. Recent strategies including covalent conjugation and label-free target identification have emerged to overcome limitations in target protein identification, leading to significant advancements in the field.

CELL CHEMICAL BIOLOGY (2021)

Review Biochemistry & Molecular Biology

Chemical Tools for Endogenous Protein Labeling and Profiling

Kazuki Shiraiwa et al.

CELL CHEMICAL BIOLOGY (2020)

Article Chemistry, Multidisciplinary

A programmable chemical switch based on triggerable Michael acceptors

Jiaming Zhuang et al.

CHEMICAL SCIENCE (2020)

Article Multidisciplinary Sciences

Single-site glycine-specific labeling of proteins

Landa Purushottam et al.

NATURE COMMUNICATIONS (2019)

Review Chemistry, Multidisciplinary

Chemistry for Covalent Modification of Endogenous/Native Proteins: From Test Tubes to Complex Biological Systems

Tomonori Tamura et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2019)

Review Pharmacology & Pharmacy

Discovery of hidden allosteric sites as novel targets for allosteric drug design

Shaoyong Lu et al.

DRUG DISCOVERY TODAY (2018)

Article Chemistry, Multidisciplinary

Chemo- and Regioselective Lysine Modification on Native Proteins

Maria J. Matos et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2018)

Article Chemistry, Multidisciplinary

Rational Design of Supramolecular Dynamic Protein Assemblies by Using a Micelle-Assisted Activity-Based Protein-Labeling Technology

Britto S. Sandanaraj et al.

CHEMISTRY-A EUROPEAN JOURNAL (2018)

Article Chemistry, Multidisciplinary

Live-Cell Protein Sulfonylation Based on Proximity-driven N-Sulfonyl Pyridone Chemistry

Kazuya Matsuo et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2018)

Article Chemistry, Multidisciplinary

Covalent Modulators of the Vacuolar ATPase

Ying-Chu Chen et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2017)

Article Chemistry, Multidisciplinary

2-Aryl-5-carboxytetrazole as a New Photoaffinity Label for Drug Target Identification

Andras Herner et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2016)

Review Chemistry, Multidisciplinary

Fishing the PTM proteome with chemical approaches using functional solid phases

Ying Zhang et al.

CHEMICAL SOCIETY REVIEWS (2015)

Review Chemistry, Medicinal

Photoaffinity labeling in target- and binding-site identification

Ewan Smith et al.

FUTURE MEDICINAL CHEMISTRY (2015)

Article Chemistry, Multidisciplinary

Live cell off-target identification of lapatinib using ligand-directed tosyl chemistry

Kei Yamaura et al.

CHEMICAL COMMUNICATIONS (2014)

Review Chemistry, Multidisciplinary

Cellular Incorporation of Unnatural Amino Acids and Bioorthogonal Labeling of Proteins

Kathrin Lang et al.

CHEMICAL REVIEWS (2014)

Article Biochemistry & Molecular Biology

LDAI-Based Chemical Labeling of Intact Membrane Proteins and Its Pulse-Chase Analysis under Live Cell Conditions

Takayuki Miki et al.

CHEMISTRY & BIOLOGY (2014)

Review Chemistry, Multidisciplinary

Target Identification for Small Bioactive Molecules: Finding the Needle in the Haystack

Slava Ziegler et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2013)

Article Biochemistry & Molecular Biology

A propofol binding site on mammalian GABAA receptors identified by photolabeling

Grace M. S. Yip et al.

NATURE CHEMICAL BIOLOGY (2013)

Article Chemistry, Multidisciplinary

Phosphopeptide-Dependent Labeling of 14-3-3?? Proteins by Fusicoccin-Based Fluorescent Probes

Michiko Takahashi et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2012)

Article Chemistry, Multidisciplinary

Ligand-Directed Acyl Imidazole Chemistry for Labeling of Membrane-Bound Proteins on Live Cells

Sho-hei Fujishima et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2012)

Article Biochemistry & Molecular Biology

Identification of a Substrate-binding Site in a Peroxisomal β-Oxidation Enzyme by Photoaffinity Labeling with a Novel Palmitoyl Derivative

Yoshinori Kashiwayama et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2010)

Review Chemistry, Multidisciplinary

Bioorthogonal Chemistry: Fishing for Selectivity in a Sea of Functionality

Ellen M. Sletten et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2009)

Article Chemistry, Multidisciplinary

Marinopyrrole A Target Elucidation by Acyl Dye Transfer

Chambers C. Hughes et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2009)

Article Biochemistry & Molecular Biology

Ligand-directed tosyl chemistry for protein labeling in vivo

Shinya Tsukiji et al.

NATURE CHEMICAL BIOLOGY (2009)

Review Chemistry, Multidisciplinary

Carbonic anhydrase as a model for biophysical and physical-organic studies of proteins and protein-ligand binding

Vijay M. Krishnamurthy et al.

CHEMICAL REVIEWS (2008)

Review Biochemistry & Molecular Biology

Searching for new allosteric sites in enzymes

JA Hardy et al.

CURRENT OPINION IN STRUCTURAL BIOLOGY (2004)

Article Chemistry, Multidisciplinary

Reactivity of functional groups on the protein surface: Development of epoxide probes for protein labeling

G Chen et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2003)