期刊
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
卷 1864, 期 2, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.bbabio.2022.148935
关键词
Light-harvesting complex; Carotenoid; TR-EPR; Emiliania huxleyi; Diatom; Photoprotection
Detailed information on photo-generated triplet states of diatom and haptophyte Fucoxanthin Chlorophyll-binding Proteins (FCPs and E-FCPs) has been obtained through a combined spectroscopic investigation. The study reveals a shared photoprotective triplet-triplet energy transfer pathway in these proteins involving a specific Fucoxanthin site.
Detailed information on the photo-generated triplet states of diatom and haptophyte Fucoxanthin Chlorophyll -binding Proteins (FCPs and E-FCPs, respectively) have been obtained from a combined spectroscopic investi-gation involving Transient Absorption and Time-Resolved Electron Paramagnetic Resonance. Pennate diatom Phaeodactylum tricornutum FCP shows identical photoprotective Triplet-Triplet Energy Transfer (TTET) pathways to the previously investigated centric diatom Cyclotella meneghiniana FCP, with the same two chlorophyll a- fucoxanthin pairs that involve the fucoxanthins in sites Fx301 and Fx302 contributing to TTET in both diatom groups. In the case of the haptophyte Emilianina huxleyi E-FCP, only one of the two chlorophyll a-fucoxanthins pairs observed in diatoms, the one involving chlorophyll a409 and Fx301, has been shown to be active in TTET. Furthermore, despite the marked change in the pigment content of E-FCP with growth light intensity, the TTET pathway is not affected. Thus, our comparative investigation of FCPs revealed a photoprotective TTET pathway shared within these classes involving the fucoxanthin in site Fx301, a site exposed to the exterior of the antenna monomer that has no equivalent in Light-Harvesting Complexes from the green lineage.
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