期刊
BIOCHEMICAL PHARMACOLOGY
卷 210, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bcp.2023.115458
关键词
AML; Transcription factor; Targeted therapy; Purine metabolism
This study found that FLT3 internal tandem duplication mutation led to aberrant upregulation of E2F1 in acute myeloid leukemia (AML) patients. Knockdown of E2F1 inhibited cell proliferation and increased cell sensitivity to chemotherapy in FLT3-ITD-positive AML cells. FLT3-ITD promoted E2F1 expression and nuclear accumulation in AML cells, enhancing purine metabolism and supporting AML cell proliferation.
Oncogene FLT3 internal tandem duplication (FLT3-ITD) mutation accounts for 30 % of acute myeloid leukaemia (AML) cases and induces transformation. Previously, we found that E2F transcription factor 1 (E2F1) was involved in AML cell differentiation. Here, we reported that E2F1 expression was aberrantly upregulated in AML patients, especially in AML patients carrying FLT3-ITD. E2F1 knockdown inhibited cell proliferation and increased cell sensitivity to chemotherapy in cultured FLT3-ITD-positive AML cells. E2F1-depleted FLT3-ITD+ AML cells lost their malignancy as shown by the reduced leukaemia burden and prolonged survival in NOD-PrkdcscidIl2rgem1/Smoc mice receiving xenografts. Additionally, FLT3-ITD-driven transformation of human CD34+ hematopoietic stem and progenitor cells was counteracted by E2F1 knockdown. Mechanistically, FLT3- ITD enhanced the expression and nuclear accumulation of E2F1 in AML cells. Further study using chromatin immunoprecipitation-sequencing and metabolomics analyses revealed that ectopic FLT3-ITD promoted the recruitment of E2F1 on genes encoding key enzymatic regulators of purine metabolism and thus supported AML cell proliferation. Together, this study demonstrates that E2F1-activated purine metabolism is a critical down-stream process of FLT3-ITD in AML and a potential target for FLT3-ITD+ AML patients.
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