MiR-28-5p Promotes Osteosarcoma Development by Suppressing URGCP Expression

IntroductionThe purpose is to investigate the role of miR-28-5p in osteosarcoma (OS) development.Materials and MethodsThe expressions of miR-28-5p and URGCP in OS tissues (n = 30) and cells (MG-63 and U2OS) were discovered by q-PCR assay. MiR-28-5p mimic, sh-URGCP, pcDNA3.1-URGCP, and their controls were transfected by lipofectamine (TM) 2000. CCK8 and tunel experiments were processed for proliferation and apoptosis testing. Migration and invasion were monitored by transwell assay. Western blot was undertaken to show the levels of Bax and Bcl-2. The target between miR-28-5p and URGCP was confirmed via a luciferase reporter gene experiment. Finally, the rescue assay further verified the function of miR-28-5p and URGCP in OS cells.ResultsMiR-28-5p expressed lower (P < 0.001) in OS tissue and cells. MiR-28-5p mimics suppressed (P < 0.05) proliferation and migration ability, and it accelerated apoptosis of osteosarcoma cells. MiR-28-5p targeted and negatively regulated URGCP expression. Sh-URGCP suppressed (P < 0.01) proliferation and migration ability, while it improved apoptosis of OS cells. Overexpression of miR-28-5p obviously accelerated (P < 0.05) Bax expression, while it reduced (P < 0.05) the Bcl-2 level. Interestingly, pcDNA3.1-URGCP rescued the process. Up-regulated URGCP rescued the effects of miR-28-5p mimic in vitro.ConclusionsMiR-28-5p accelerates the proliferation and migration of osteosarcoma cells, and inhibits tumor cell apoptosis by suppressing URGCP expression, which could be regarded as a potential target for OS treatment.

Automated summary

This study investigates the role of miR-28-5p in the development of osteosarcoma. The results show that miR-28-5p suppresses URGCP expression, leading to accelerated proliferation and migration of osteosarcoma cells and inhibiting apoptosis. This suggests that miR-28-5p could be a potential target for treating osteosarcoma.